Human/Mouse Sonic Hedgehog/Shh N-Terminus PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Sonic Hedgehog/SHH in Western blots and mouse Sonic Hedgehog/SHH in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Desert Hedgehog/Dhh (N‑terminus; aa 23-198) is observed and no cross-reactivity with rmIndian Hedgehog/Ihh (N-terminus; aa 66-240), rmDesert Hedgehog/Dhh (C‑terminus; aa 199-396), or rmSonic Hedgehog/Shh (C‑terminus; aa 199-437) is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 171018
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Sonic Hedgehog/SHH N-terminus
    Cys25-Gly198 (Lys122Arg)
    Accession # Q62226
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Sonic Hedgehog/Shh in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus PE‑conjugated Monoclonal Antibody (Catalog # IC4641P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

  • Entrez Gene IDs:
    6469 (Human); 20423 (Mouse)
  • Alternate Names:
    HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; sonic hedgehog; TPT; TPTPS
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Canonical and noncanonical Hedgehog pathway in the pathogenesis of multiple myeloma.
    Authors: Blotta, Simona, Jakubikova, Jana, Calimeri, Teresa, Roccaro, Aldo M, Amodio, Nicola, Azab, Abdel Ka, Foresta, Umberto, Mitsiades, Constant, Rossi, Marco, Todoerti, Katia, Molica, Stefano, Morabito, Fortunat, Neri, Antonino, Tagliaferri, Piersand, Tassone, Pierfran, Anderson, Kenneth, Munshi, Nikhil C
    Blood, 2012;120(25):5002-13.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  2. Role for hedgehog pathway in regulating growth and function of invariant NKT cells.
    Authors: Syn WK, Witek RP, Curbishley SM, Jung Y, Choi SS, Enrich B, Omenetti A, Agboola KM, Fearing CM, Tilg H, Adams DH, Diehl AM
    Eur. J. Immunol., 2009;39(7):1879-92.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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