Mouse Sonic Hedgehog/Shh N-Terminus Antibody

Catalog #: MAB4641
2 Citations Datasheet / COA / SDS

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MAB4641-SP
MAB4641-100
MAB4641-500

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Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody.

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Citations (2)

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Mouse Sonic Hedgehog/Shh N-Terminus Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh
(N‑terminus; aa 23‑198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66‑240), rmDhh (C‑terminus; aa 199‑396), or rmShh (C‑terminus; aa 199-437) is observed.

Source

Monoclonal Rat IgG_2A Clone # 171018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog
Cys25-Gly198 (Lys122Arg)
Accession # Q62226

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Mouse Sonic Hedgehog Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody (Catalog # BAM4642)

Standard: Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein (Catalog # 461-SH)

Neutralization

Measured by its ability to neutralize Sonic Hedgehog-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 5-15 µg/mL in the presence of 5 µg/mL Recombinant Mouse Sonic Hedgehog N‑Terminus.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody. Recombinant Mouse Sonic Hedgehog N-Terminus (Catalog # 461-SH or 464-SH) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N-Terminus (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Sonic Hedgehog N-Terminus Monoclonal Antibody (Catalog # MAB4641). The ND₅₀ is typically 5-15 µg/mL.

Western Blot

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Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Sonic Hedgehog/Shh in Mouse Embryo. Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Alternate Names

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse Sonic Hedgehog/Shh N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair.
Authors: Engevik A, Feng R, Yang L, Zavros Y
Endocrinology, 2013-10-03;154(12):4627-39.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development.
Authors: Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L
Stem Cells, 2007-02-01;25(5):1156-65.
Species: Mouse
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Neutralization