Human/Mouse SorLA Antibody

Catalog # Availability Size / Price Qty
AF5699
AF5699-SP
Detection of Mouse SorLA by Western Blot.
2 Images
Product Details
Citations (1)
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Human/Mouse SorLA Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SorLA in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse SorLA
Ser82-Asn367
Accession # Q92673
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse SorLA antibody by Western Blot. View Larger

Detection of Mouse SorLA by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5699) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorLA at approximately 330 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SorLA antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

SorLA in Human Brain. SorLA was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human/Mouse SorLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5699) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorLA

SorLA (Sorting protein-related receptor containing LDLR class A repeats; also LR11 and SorL1) is a 250-330 kDa, monomeric member of the SORL1 family of proteins. It is expressed on the cell surface (10%) and in Golgi/ER membranes (90%) of neurons and vascular smooth muscle cells. SorLA binds apoE-rich lipoproteins plus the 39 kDa RAP. It also acts as an intracellular sorting receptor for APP. In this context, it blocks transport of APP into compartments that exhibit beta -secretase activity, thus interfering with A beta peptide generation. Human SorLA is a type I transmembrane glycoprotein 2214 amino acids (aa) in length. It contains a 28 aa signal sequence, a 53 aa propeptide, and a 2133 aa mature molecule. The mature molecule contains a 2056 aa extracellular domain (ECD) (aa 82-2137) and a short 56 aa cytoplasmic tail. The ECD is highly modular, containing five BNR/Asp-box repeats (aa 136-573), five LDLR class B repeats (aa 800-1013), one EGF-like domain (aa 1026‑1072), 11 LDLR class A domains (aa 1076-1551) and six FN type III repeats (aa 1554-2023). Multiple potential splice variants exist. There are alternative start sites at Met1057 and Met1155, plus a 63 aa substitution for aa 1-1153 and a 19 aa substitution for aa 1-1404. Over aa 82-367, human SorLA shares 93% aa identity with mouse SorLA.

Long Name
Sortilin-related Receptor Containing LDLR Class A Repeats
Entrez Gene IDs
6653 (Human); 20660 (Mouse); 300652 (Rat)
Alternate Names
C11orf32; chromosome 11 open reading frame 32; FLJ21930; FLJ39258; gp250; Low-density lipoprotein receptor relative with 11 ligand-binding repeats; LR11; LR11LDLR relative with 11 ligand-binding repeats; LRP9; mosaic protein LR11; SORL1; SorLA; SorLA-1SORLA; sortilin-related receptor; sortilin-related receptor, L(DLR class) A repeats containing; sortilin-related receptor, L(DLR class) A repeats-containing; Sorting protein-related receptor containing LDLR class A repeats

Product Datasheets

Citation for Human/Mouse SorLA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017;13(6):e1006470.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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