Human/Mouse TIAM1 Antibody

Catalog # Availability Size / Price Qty
AF5038
AF5038-SP
Detection of Human/Mouse TIAM1 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse TIAM1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse TIAM1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TIAM1
Met1-Ala378
Accession # Q13009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse TIAM1 antibody by Western Blot. View Larger

Detection of Human/Mouse TIAM1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIAM1

TIAM1 (T lymphoma invasion and metastasis) is an approximately 200 kDa member of the TIAM family of guanine-nucleotide exchange factors. It is expressed in multiple cell types, including endothelial cells, neurons, Schwann cells and almost all tumors. TIAM1 stimulates the activity of RHO-like GTPases and thus connects extracellular signals to the cytoskeleton. Human TIAM1 is 1591 amino acids (aa) in length. It contains a myristoylation site at Gly2, plus two pleckstrin homology domains (aa 434 - 549 and 1261 - 1397), a Ras-binding region (aa 765 - 832) and a RhoGEF/DH domain (aa 1041 - 1232). Over aa 1 - 378, human TIAM1 shows 94% aa identity to mouse TIAM1.

Long Name
T-cell Lymphoma Invasion and Metastasis 1
Entrez Gene IDs
7074 (Human); 21844 (Mouse); 304109 (Rat)
Alternate Names
FLJ36302; human T-lymphoma invasion and metastasis inducing TIAM1 protein, 10TIAM-1; T-cell lymphoma invasion and metastasis 1; TIAM1; T-lymphoma invasion and metastasis-inducing protein 1

Product Datasheets

Citations for Human/Mouse TIAM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. P-REX1-Independent, Calcium-Dependent RAC1 Hyperactivation in Prostate Cancer
    Authors: MJ Baker, MC Abba, R Garcia-Mat, MG Kazanietz
    Cancers (Basel), 2020;12(2):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  2. Tiam1 is critical for glutamatergic synapse structure and function in the hippocampus
    Authors: S Rao, Y Kay, BE Herring
    J. Neurosci., 2019;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells
    Authors: Z Diamantopo, G White, MZH Fadlullah, M Dreger, K Pickering, J Maltas, G Ashton, R MacLeod, GS Baillie, V Kouskoff, G Lacaud, GI Murray, OJ Sansom, AFL Hurlstone, A Malliri
    Cancer Cell, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway.
    Authors: Yuan Wang, Feng Yan, Qing Ye, Xiao Wu, Fan Jiang
    Scientific Reports, 2016;0(0):2045-2322.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Reviews for Human/Mouse TIAM1 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
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3 Star
66.67%
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Human/Mouse TIAM1 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Mouse TIAM1 Antibody
By Anonymous on 08/03/2018
Application: ICC/IF Sample Tested: HCC44 Species: Human

4% PFA fixation
1:100 dilution


Human/Mouse TIAM1 Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: MCF-7 human breast cancer cell line,SK-BR-3 human breast cancer cell line Species: Human

Total cell lysates from MCF-7 and SK-BR-3 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human TIAM1 Antibody (AF5038). A specific band was detected for TIAM1 at approximately 180 kDa. This experiment was conducted under reducing conditions.