Human/Mouse TIAM1 Antibody

R&D Systems | Catalog # AF5038

R&D Systems
Discontinued Product
AF5038 has been discontinued. View all TIAM1 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TIAM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TIAM1
Met1-Ala378
Accession # Q13009

Specificity

Detects endogenous human and mouse TIAM1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse TIAM1 Antibody

Detection of Human/Mouse TIAM1 antibody by Western Blot.

Detection of Human/Mouse TIAM1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse TIAM1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: D3 mouse embryonic stem cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line

Reviewed Applications

Read 3 reviews rated 3.7 using AF5038 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIAM1

TIAM1 (T lymphoma invasion and metastasis) is an approximately 200 kDa member of the TIAM family of guanine-nucleotide exchange factors. It is expressed in multiple cell types, including endothelial cells, neurons, Schwann cells and almost all tumors. TIAM1 stimulates the activity of RHO-like GTPases and thus connects extracellular signals to the cytoskeleton. Human TIAM1 is 1591 amino acids (aa) in length. It contains a myristoylation site at Gly2, plus two pleckstrin homology domains (aa 434 - 549 and 1261 - 1397), a Ras-binding region (aa 765 - 832) and a RhoGEF/DH domain (aa 1041 - 1232). Over aa 1 - 378, human TIAM1 shows 94% aa identity to mouse TIAM1.

Long Name

T-cell Lymphoma Invasion and Metastasis 1

Alternate Names

FLJ36302, human T-lymphoma invasion and metastasis inducing TIAM1 protein, 10TIAM-1, T-cell lymphoma invasion and metastasis 1, T-lymphoma invasion and metastasis-inducing protein 1

Entrez Gene IDs

7074 (Human); 21844 (Mouse); 304109 (Rat)

Gene Symbol

TIAM1

UniProt

Q13009

Additional TIAM1 Products

Product Documents for Human/Mouse TIAM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse TIAM1 Antibody

For research use only

Citations for Human/Mouse TIAM1 Antibody

Customer Reviews for Human/Mouse TIAM1 Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
0%
3 Stars
67%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse TIAM1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018
  • Human/Mouse TIAM1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HCC44
    Species: Human
    Verified Customer | Posted 08/03/2018
    4% PFA fixation 1:100 dilution
    Human/Mouse TIAM1 Antibody AF5038
  • Human/Mouse TIAM1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line and SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/23/2018
    Total cell lysates from MCF-7 and SK-BR-3 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human TIAM1 Antibody (AF5038). A specific band was detected for TIAM1 at approximately 180 kDa. This experiment was conducted under reducing conditions.
    Human/Mouse TIAM1 Antibody AF5038

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