Human/Mouse TIAM1 Antibody
Human/Mouse TIAM1 Antibody Summary
Met1-Ala378
Accession # Q13009
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse TIAM1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIAM1
TIAM1 (T lymphoma invasion and metastasis) is an approximately 200 kDa member of the TIAM family of guanine-nucleotide exchange factors. It is expressed in multiple cell types, including endothelial cells, neurons, Schwann cells and almost all tumors. TIAM1 stimulates the activity of RHO-like GTPases and thus connects extracellular signals to the cytoskeleton. Human TIAM1 is 1591 amino acids (aa) in length. It contains a myristoylation site at Gly2, plus two pleckstrin homology domains (aa 434 - 549 and 1261 - 1397), a Ras-binding region (aa 765 - 832) and a RhoGEF/DH domain (aa 1041 - 1232). Over aa 1 - 378, human TIAM1 shows 94% aa identity to mouse TIAM1.
Product Datasheets
Citations for Human/Mouse TIAM1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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TIAM1-RAC1 promote small-cell lung cancer cell survival through antagonizing Nur77-induced BCL2 conformational change
Authors: A Payapilly, R Guilbert, T Descamps, G White, P Magee, C Zhou, A Kerr, KL Simpson, F Blackhall, C Dive, A Malliri
Cell Reports, 2021-11-09;37(6):109979.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
P-REX1-Independent, Calcium-Dependent RAC1 Hyperactivation in Prostate Cancer
Authors: MJ Baker, MC Abba, R Garcia-Mat, MG Kazanietz
Cancers (Basel), 2020-02-19;12(2):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Tiam1 is critical for glutamatergic synapse structure and function in the hippocampus
Authors: S Rao, Y Kay, BE Herring
J. Neurosci., 2019-10-09;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells
Authors: Z Diamantopo, G White, MZH Fadlullah, M Dreger, K Pickering, J Maltas, G Ashton, R MacLeod, GS Baillie, V Kouskoff, G Lacaud, GI Murray, OJ Sansom, AFL Hurlstone, A Malliri
Cancer Cell, 2017-04-13;0(0):.
Species: Human
Sample Types: Cell Lysates, Protein, Whole Cells, Whole Tissue
Applications: ICC, IHC, Immunoprecipitation, Western Blot -
PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway.
Authors: Yuan Wang, Feng Yan, Qing Ye, Xiao Wu, Fan Jiang
Scientific Reports, 2016-04-07;0(0):2045-2322.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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4% PFA fixation
1:100 dilution
Total cell lysates from MCF-7 and SK-BR-3 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human TIAM1 Antibody (AF5038). A specific band was detected for TIAM1 at approximately 180 kDa. This experiment was conducted under reducing conditions.
