Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Infared Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 303728
View all TOR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345

Specificity

Detects human TOR in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse TOR Antibody

TOR antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

TOR in MCF-7 Human Cell Line.

TOR was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human/Mouse TOR Monoclonal Antibody (Catalog # MAB1537) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse TOR Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB1537 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TOR

TOR, also known as FRAP1, is a member of the PI 3-kinase-related kinase (PIKK) family. It is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Entrez Gene IDs

2475 (Human); 287871 (Rat)

Gene Symbol

MTOR

UniProt

P42345

Additional TOR Products

Product Documents for Human/Mouse TOR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse TOR Antibody

For research use only

Citations for Human/Mouse TOR Antibody

Customer Reviews for Human/Mouse TOR Antibody (1)

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1 Customer Rating
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  • Human/Mouse TOR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: rat tissues
    Species: Rat
    Verified Customer | Posted 07/29/2017
    The western blot was utilized to detect the expression of mTOR
    The anti-mTOR antibody was used primary antibodies (1 : 1000; Novus Biologicals, USA).Our data showed it worked well.Antibody is good.
    Human/Mouse TOR Antibody MAB1537

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