Human/Mouse Wnt-3a Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Wnt-3a in direct ELISAs.
  • Source
    Monoclonal Rat IgG1 Clone # 930769
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-3a
    Ser19-Lys352
    Accession # P27467
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize Wnt-3a induced Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 0.5 μg/mL Recombinant Mouse Wnt-3a.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Topflash Reporter Activity Induced byWnt-3a and Neutralization by Mouse Wnt-3a Antibody. Recombinant Mouse Wnt-3a (Catalog #
1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Human Wnt-3a (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND50 is typically
0.15‑0.9 μg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

  • References:
    1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
    2. Miller, J.R. (2001) Genome Biol. 3:3001.
    3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
    4. Lu, W et al. (2004) Cell 119:97.
    5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
    6. Willert, K. et al. (2003) Nature 423:448.
    7. Komekado, H. et al. (2007) Genes Cells 12:521.
    8. Takada, R. et al. (2006) Dev. Cell 11:791.
    9. Entrez Accession # NP_001100475.
    10. Dunty, W.C. et al. (2008) Development 135:85.
    11. Lee, S.M.K. et al. (2000) Development 127:457.
    12. Takada, S. et al. (1994) Genes Dev. 8:174.
    13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
    14. Nusse, R. (2008) Cell Res. 18:523.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 3a
  • Entrez Gene IDs:
    89780 (Human); 22416 (Mouse)
  • Alternate Names:
    MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a
Related Research Areas
Isotype Controls
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Rat IgG1 Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG1 Isotype Control

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Secondary Antibodies
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115
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