Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all XIAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497 (Ser162Cys)
Accession # P98170

Specificity

Detects human and mouse XIAP in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse XIAP Biotinylated Antibody

Detection of Human and Mouse XIAP antibody by Western Blot.

Detection of Human and Mouse XIAP by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human XIAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8221) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse XIAP Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Alternate Names

BIRC4

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Gene Symbol

XIAP

UniProt

P98170

Additional XIAP Products

Product Documents for Human/Mouse XIAP Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse XIAP Biotinylated Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

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