Human/Mouse/Rat XIAP Antibody

(11 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat XIAP in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human XIAP
    Met1-Ser497 (Ser162Cys)
    Accession # P98170
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
XIAP in Human Lymphoma.

XIAP was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry
XIAP in Human Breast Cancer Tissue. XIAP was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human/Mouse/Rat XIAP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human and Mouse XIAP by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for XIAP at approximately 59 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

  • Long Name:
    X-linked Inhibitor of Apoptosis
  • Entrez Gene IDs:
    331 (Human); 11798 (Mouse); 63879 (Rat)
  • Alternate Names:
    API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
    Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival
    Authors: HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens
    PLoS ONE, 2017;12(2):e0172567.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  3. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016;21(8):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study
    Onco Targets Ther, 2016;9(0):2601-13.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
    Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
    Anticancer Res, 2014;34(12):7007-20.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders.
    Authors: Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T
    Int J Oncol, 2014;45(1):419-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Admixture fine-mapping in African Americans implicates XAF1 as a possible sarcoidosis risk gene.
    Authors: Levin A, Iannuzzi M, Montgomery C, Trudeau S, Datta I, Adrianto I, Chitale D, McKeigue P, Rybicki B
    PLoS ONE, 2014;9(3):e92646.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh
  8. Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
    Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla
    J Invest Dermatol, 2011;131(11):2327-9.
    Species: Human
    Sample Type: Serum
    Application: WB
  9. Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3.
    Authors: Lane D, Cote M, Grondin R, Couture MC, Piche A
    Mol. Cancer Ther., 2006;5(3):509-21.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  10. Involvement of the Edar signaling in the control of hair follicle involution (catagen).
    Authors: Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
    Am. J. Pathol., 2006;169(6):2075-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  11. Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications.
    Authors: Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC
    Oncogene, 2002;21(37):5673-83.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 11 Citations
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