|Detection of MUC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human MUC‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB6298P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
MUC-1 (Mucin-1; also PEM, PEMT, Episialin, Tumor-associated Mucin, and Carcinoma-associated Mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O‑glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.
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