Detection of MyD88 in Raji Human Cell Line by Flow Cytometry.
Raji human Burkitt's lymphoma cell line was stained with Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB29281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myeloid Differentiation primary response gene 88 (MyD88) contains a cytoplasmic Toll/interleukin 1 receptor (TIR) and functions as an adaptor protein in Toll-like receptor signaling. It is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.
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