Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody

Catalog # Availability Size / Price Qty
MAB5469
MAB5469-SP
Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot.
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Product Details
Citations (5)
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Reviews (1)

Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 706824
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Leu189-Leu741
Accession # Q09328
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human N-Acetylglucosaminyltransferase V/MGAT5 antibody by Western Blot. View Larger

Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry N-Acetylglucosaminyltransferase V/MGAT5 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

N‑Acetylglucosaminyltransferase V/MGAT5 in MCF‑7 Human Cell Line. N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: N-Acetylglucosaminyltransferase V/MGAT5

N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha 1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta 1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).

References
  1. Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
  2. Lau, K.S. et al. (2007) Cell 198:123.
  3. Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
  4. Granovsky, M. et al. (2008) Nat. Med. 7:1.
  5. Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.
Long Name
alpha-1,6-Mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Entrez Gene IDs
4249 (Human); 107895 (Mouse); 65271 (Rat)
Alternate Names
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; EC 2.4.1.155; GGNT5; GlcNAc-T V; GNT-VGNT-VA; Mannoside acetylglucosaminyltransferase 5; mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; MGAT5; N-Acetylglucosaminyltransferase V; N-acetylglucosaminyl-transferase V

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Citations for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan
    Authors: Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
    Communications Biology
  2. N-Acetylglucosaminyltransferase-V requires a specific non-catalytic luminal domain for its activity toward glycoprotein substrates
    Authors: RF Osuka, T Hirata, M Nagae, M Nakano, H Shibata, R Okamoto, Y Kizuka
    The Journal of Biological Chemistry, 2022-01-30;0(0):101666.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased beta1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding.
    Authors: Priglinger C, Obermann J, Szober C, Merl-Pham J, Ohmayer U, Behler J, Gruhn F, Kreutzer T, Wertheimer C, Geerlof A, Priglinger S, Hauck S
    PLoS ONE, 2016-01-13;11(1):e0146887.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
    Authors: Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R
    EMBO J, 2014-10-29;33(24):2890-905.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells
    Authors: Tetsuya Hirata, Yoichiro Harada, Koichiro M. Hirosawa, Yuko Tokoro, Kenichi G.N. Suzuki, Yasuhiko Kizuka
    iScience

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Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
By SOK LEE on 03/23/2018
Application: WB Sample Tested: Cell Lysates Species: Human

MGAT5 western blotting after MGAT5A transfection to 293 cells
(Figure Legend)
Ctrl: Control vector was transfected for 48 hrs.
MGAT5: MGAT5A vector was transfected for 48 hrs.
Above blots: human MGAT5 (80-100 kDa)
Lower blots: beta-actin (42-kDa)
Western blot condition: Tris buffer (0.5% Triton X)-lysed cell extract (15 ug each) was loaded with non-reducing condition (:No beta-MercaptoEthanol on loading buffer).
Primary Ab: anti-MGAT5 antibody (1 ug/ml) overnight at 4C
Secondary Ab: anti-mouse HRP antibody (1:10000 dilution) 90 minute at room temperature.
Development: SuperSignal West-Pico PLUS for 15 sec.