Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody

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Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot.
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Citations (5)
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Reviews (1)

Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody Summary

Species Reactivity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2A Clone # 706824
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Accession # Q09328
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human N-Acetylglucosaminyltransferase V/MGAT5 antibody by Western Blot. View Larger

Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry N-Acetylglucosaminyltransferase V/MGAT5 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

N‑Acetylglucosaminyltransferase V/MGAT5 in MCF‑7 Human Cell Line. N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: N-Acetylglucosaminyltransferase V/MGAT5

N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha 1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta 1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).

  1. Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
  2. Lau, K.S. et al. (2007) Cell 198:123.
  3. Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
  4. Granovsky, M. et al. (2008) Nat. Med. 7:1.
  5. Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.
Long Name
alpha-1,6-Mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Entrez Gene IDs
4249 (Human); 107895 (Mouse); 65271 (Rat)
Alternate Names
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; EC; GGNT5; GlcNAc-T V; GNT-VGNT-VA; Mannoside acetylglucosaminyltransferase 5; mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; MGAT5; N-Acetylglucosaminyltransferase V; N-acetylglucosaminyl-transferase V

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Citations for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan
    Authors: Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
    Communications Biology
  2. N-Acetylglucosaminyltransferase-V requires a specific non-catalytic luminal domain for its activity toward glycoprotein substrates
    Authors: RF Osuka, T Hirata, M Nagae, M Nakano, H Shibata, R Okamoto, Y Kizuka
    The Journal of Biological Chemistry, 2022-01-30;0(0):101666.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased beta1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding.
    Authors: Priglinger C, Obermann J, Szober C, Merl-Pham J, Ohmayer U, Behler J, Gruhn F, Kreutzer T, Wertheimer C, Geerlof A, Priglinger S, Hauck S
    PLoS ONE, 2016-01-13;11(1):e0146887.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
    Authors: Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R
    EMBO J, 2014-10-29;33(24):2890-905.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells
    Authors: Tetsuya Hirata, Yoichiro Harada, Koichiro M. Hirosawa, Yuko Tokoro, Kenichi G.N. Suzuki, Yasuhiko Kizuka


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Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
By SOK LEE on 03/23/2018
Application: WB Sample Tested: Cell Lysates Species: Human

MGAT5 western blotting after MGAT5A transfection to 293 cells
(Figure Legend)
Ctrl: Control vector was transfected for 48 hrs.
MGAT5: MGAT5A vector was transfected for 48 hrs.
Above blots: human MGAT5 (80-100 kDa)
Lower blots: beta-actin (42-kDa)
Western blot condition: Tris buffer (0.5% Triton X)-lysed cell extract (15 ug each) was loaded with non-reducing condition (:No beta-MercaptoEthanol on loading buffer).
Primary Ab: anti-MGAT5 antibody (1 ug/ml) overnight at 4C
Secondary Ab: anti-mouse HRP antibody (1:10000 dilution) 90 minute at room temperature.
Development: SuperSignal West-Pico PLUS for 15 sec.