Human N-Cadherin Biotinylated Antibody

Catalog #: BAF1388
1 Citation Datasheet / COA / SDS

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human N-Cadherin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human N-Cadherin Propeptide in Western blots. In this format, less than 5% cross-reactivity with recombinant human (rh) N‑Cadherin extracellular domain, rhCadherin-8, rhCadherin-11, and rhR-Cadherin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human N-Cadherin Propeptide
Ser26-Arg159
Accession # P19022

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human N-Cadherin Propeptide

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: N-Cadherin

N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130 ‑ 135 kDa member of the “classical” (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15 ‑ 20 kDa prodomain (aa 26 ‑ 159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N-Cadherin proregion.

Long Name

Neural Cadherin

Entrez Gene IDs

1000 (Human); 12558 (Mouse); 83501 (Rat)

Alternate Names

ACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325 antigen; CD325; CDH2; CDHN; CDw325; NCAD; N-cadherin 1; NCadherin; N-Cadherin; Neural cadherin; neural-cadherin

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human N-Cadherin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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