Detection of Nectin‑1 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Nectin‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB2880A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).
Lopez, M. et al. (1995) Gene 155:261.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
Lopez, M. et al. (2001) J. Virol. 75:5684.
Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
Linehan, M. M. et al. (2004) J. Virol. 78:2530.
Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
Togashi, H. et al. (2006) J. Cell Biol. 174:141.
Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Suzuki, K. et al. (2000) Nat. Genet. 25:427.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
Have you used Human Nectin-1 APC-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.