Human Nectin-1 APC-conjugated Antibody

FAB2880A has been discontinued. View all Nectin-1 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Nectin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Nectin-2, 3, 4, or recombinant mouse Nectin-1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 610835
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Nectin-1
    Gln31-Thr334
    Accession # Q15223
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Nectin‑1 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Nectin‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB2880A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nectin-1

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).

  • References:
    1. Lopez, M. et al. (1995) Gene 155:261.
    2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
    3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
    4. Lopez, M. et al. (2001) J. Virol. 75:5684.
    5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
    6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
    7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
    8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
    9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
    10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
    11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
    12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
    13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
    14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  • Long Name:
    Poliovirus Receptor Related 1
  • Entrez Gene IDs:
    5818 (Human); 58235 (Mouse)
  • Alternate Names:
    CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12
Related Research Areas

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