Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 196908
View all Nestin Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Nestin
Accession # P48681

Specificity

Detects human Nestin in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Nestin Fluorescein‑conjugated Antibody

Detection of Nestin antibody in NTera‑2 Human Cell Line antibody by Flow Cytometry.

Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1259F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Nestin Fluorescein‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 1 using IC1259F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Nestin

Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in both the neural differentiation pathway and that of CNS glia. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Full-length human Nestin shares 51% amino acid sequence identity to mouse Nestin.

Alternate Names

NES

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Gene Symbol

NES

UniProt

P48681

Additional Nestin Products

Product Documents for Human Nestin Fluorescein‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Nestin Fluorescein‑conjugated Antibody

For research use only

Citations for Human Nestin Fluorescein‑conjugated Antibody

Customer Reviews for Human Nestin Fluorescein‑conjugated Antibody (1)

1 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
100%

Have you used Human Nestin Fluorescein‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human Nestin Fluorescein-conjugated Antibody
    Name: Scott Schachtele
    Application: Flow Cytometry
    Sample Tested: Neural progenitor cells and Undifferentiated neural progenitor cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    We wanted to use this antibody to study expression of our fetal-derived neural stem cell lines in flow cytometry. We were not able to determine an appropriate antibody dilution using positive and negative control cell lines because the negative control line stained positive at all dilutions.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies