Human Nestin PE-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Nestin.
  • Source
    Monoclonal Mouse IgG1 Clone # 196908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Nestin
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 μM Retinoic Acid for 60 days (light orange filled histogram) was stained with Mouse Anti-Human Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC1259P) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nestin
Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  • References:
    1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
    2. Lendahl, U. et al. (1990) Cell 60:585.
    3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
    4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
    5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
    6. Frederiksen, K. et al. (1988) Neuron 1:439.
    7. Cattaneo, E. et al. (1990) Nature 347:762.
    8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
    9. Rietze, R.L. et al. (2001) Nature 412:736.
    10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
    11. Zulewski, H. et al. (2001) Diabetes 50:521.
    12. Lumelsky, N. et al. (2001) Science 292:1389.
    13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
    14. Shih, C.C. et al. (2001) Blood 98:2412.
  • Entrez Gene IDs:
    10763 (Human); 18008 (Mouse); 25491 (Rat)
  • Alternate Names:
    FLJ21841; NES; Nestin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Phenotypic diversity of patient-derived melanoma populations in stem cell medium.
    Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M
    Lab Invest, 2015;95(6):672-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.
    Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
    J. Invest. Dermatol., 2011;131(2):487-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. MDR1 expression identifies human melanoma stem cells.
    Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
    Biochem. Biophys. Res. Commun., 2008;368(4):930-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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