Human Nestin Antibody

(46 citations)
(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Nestin.
  • Source
    Monoclonal Mouse IgG1 Clone # 196908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Nestin
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-reported
    This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Nestin in Human Neural Progenitor Cells. Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nestin
Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  • References:
    1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
    2. Lendahl, U. et al. (1990) Cell 60:585.
    3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
    4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
    5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
    6. Frederiksen, K. et al. (1988) Neuron 1:439.
    7. Cattaneo, E. et al. (1990) Nature 347:762.
    8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
    9. Rietze, R.L. et al. (2001) Nature 412:736.
    10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
    11. Zulewski, H. et al. (2001) Diabetes 50:521.
    12. Lumelsky, N. et al. (2001) Science 292:1389.
    13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
    14. Shih, C.C. et al. (2001) Blood 98:2412.
  • Entrez Gene IDs:
    10763 (Human); 18008 (Mouse); 25491 (Rat)
  • Alternate Names:
    FLJ21841; NES; Nestin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Induction of senescence in primary glioblastoma cells by serum and TGF?
    Authors: R Kumar, A Gont, TJ Perkins, JEL Hanson, IAJ Lorimer
    Sci Rep, 2017;7(1):2156.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
    PLoS ONE, 2017;12(4):e0174074.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells
    Authors: HO King, T Brend, HL Payne, A Wright, TA Ward, K Patel, T Egnuni, LF Stead, A Patel, H Wurdak, SC Short
    Stem Cell Reports, 2017;8(1):125-139.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene
    Authors: SB Ross, ST Fraser, N Nowak, C Semsarian
    Stem Cell Res, 2017;20(0):88-90.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
    Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
    BMC Cancer, 2017;17(1):178.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  6. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017;19(1):20-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
    Authors: AM Rosager, MD Sørensen, RH Dahlrot, S Hansen, DL Schonberg, JN Rich, JD Lathia, BW Kristensen
    PLoS ONE, 2017;12(8):e0182954.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC paraffin embedded
  9. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype
    PLoS ONE, 2016;11(5):e0155106.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  10. BIX01294, an inhibitor of histone methyltransferase, induces autophagy-dependent differentiation of glioma stem-like cells
    Sci Rep, 2016;6(0):38723.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  11. Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and�Motility
    Stem Cell Reports, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  12. Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors
    PLoS ONE, 2016;11(12):e0169129.
    Species: Human
    Sample Type: Whole Tissue
    Application: ICC
  13. Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
    Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
    Stem Cells, 2015;33(4):1142-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. The evidence of glioblastoma heterogeneity.
    Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M
    Sci Rep, 2015;5(0):7979.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT).
    Authors: Jeitany M, Pineda J, Liu Q, Porreca R, Hoffschir F, Desmaze C, Silvestre D, Mailliet P, Junier M, Londono-Vallejo A, Segal-Bendirdjian E, Chneiweiss H, Boussin F
    Int J Cancer, 2015;136(7):1546-58.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Frozen
  16. Phenotypic plasticity in normal breast derived epithelial cells.
    Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
    BMC Cell Biol, 2014;15(0):20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Frozen
  17. Epigenetic silencing of miRNA-9 is associated with HES1 oncogenic activity and poor prognosis of medulloblastoma.
    Authors: Fiaschetti G, Abela L, Nonoguchi N, Dubuc A, Remke M, Boro A, Grunder E, Siler U, Ohgaki H, Taylor M, Baumgartner M, Shalaby T, Grotzer M
    Br J Cancer, 2014;110(3):636-47.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Fresh
  18. Molecular evidence for OCT4-induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors.
    Authors: Mitchell R, Szabo E, Shapovalova Z, Aslostovar L, Makondo K, Bhatia M
    Stem Cells, 2014;32(8):2178-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
    Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
    Int J Oncol, 2014;44(4):1118-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  20. Novel anti-glioblastoma agents and therapeutic combinations identified from a collection of FDA approved drugs.
    Authors: Jiang, Pengfei, Mukthavaram, Rajesh, Chao, Ying, Bharati, Ila Sri, Fogal, Valentin, Pastorino, Sandra, Cong, Xiuli, Nomura, Natsuko, Gallagher, Matt, Abbasi, Taher, Vali, Shireen, Pingle, Sandeep, Makale, Milan, Kesari, Santosh
    J Transl Med, 2014;12(0):13.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  21. Nestin is highly expressed in advanced-stage melanomas and neurotized nevi.
    Authors: Akiyama, Michiko, Matsuda, Yoko, Ishiwata, Toshiyuk, Naito, Zenya, Kawana, Seiji
    Oncol Rep, 2013;29(4):1595-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  22. Activation of the type I interferon pathway is enhanced in response to human neuronal differentiation.
    Authors: Farmer, Jocelyn, Altschaefl, Kate M, O'Shea, K Sue, Miller, David J
    PLoS ONE, 2013;8(3):e58813.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  23. Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
    Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
    PLoS ONE, 2013;8(3):e59252.
  24. Embryonic stem cell-based modeling of tau pathology in human neurons.
    Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P
    Am J Pathol, 2013;182(5):1769-79.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  25. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012;7(5):e35639.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  26. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  27. Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes.
    Authors: Engelen E, Akinci U, Bryne JC
    Nat. Genet., 2011;43(6):607-11.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  28. Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.
    Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
    J. Invest. Dermatol., 2011;131(2):487-94.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  29. Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling.
    Authors: Yuki K, Natsume A, Yokoyama H, Kondo Y, Ohno M, Kato T, Chansakul P, Ito M, Kim SU, Wakabayashi T
    Cancer Lett., 2009;284(1):71-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  30. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  31. Glial cell type-specific subcellular localization of 14-3-3 zeta: an implication for JCV tropism.
    Authors: Lamba S, Ravichandran V, Major EO
    Glia, 2009;57(9):971-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  32. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture.
    Authors: Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, Smith A
    Mol. Cell. Neurosci., 2008;38(2):245-58.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  33. Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures.
    Authors: Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V
    Invest. Ophthalmol. Vis. Sci., 2008;49(12):5325-31.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  34. The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6.
    Authors: Hattermann K, Ludwig A, Gieselmann V, Held-Feindt J, Mentlein R
    Mol. Cell. Neurosci., 2008;39(1):133-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  35. Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.
    Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O
    Stem Cells, 2008;26(7):1705-12.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  36. Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions.
    Authors: Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T
    Biochem. Biophys. Res. Commun., 2007;361(3):586-92.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  37. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
    Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
    Exp. Hematol., 2007;35(7):1119-31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  38. The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress.
    Authors: Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J
    Neurosci. Lett., 2007;423(3):225-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  39. Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue.
    Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD
    Stem Cells, 2007;25(5):1298-306.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  40. Impaired proliferation and migration in human Miller-Dieker neural precursors.
    Authors: Sheen VL, Ferland RJ, Harney M, Hill RS, Neal J, Banham AH, Brown P, Chenn A, Corbo J, Hecht J, Folkerth R, Walsh CA
    Ann. Neurol., 2006;60(1):137-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  41. Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions.
    Authors: Shin S, Mitalipova M, Noggle S, Tibbitts D, Venable A, Rao R, Stice SL
    Stem Cells, 2006;24(1):125-38.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  42. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  43. Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages.
    Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N
    Br. J. Haematol., 2006;135(5):703-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  44. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004;152(2):159-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  45. Differentiation of human embryonic stem cells into insulin-producing clusters.
    Authors: Segev H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J
    Stem Cells, 2004;22(3):265-74.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  46. Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells.
    Authors: Gerecht-Nir S, Cohen S, Ziskind A, Itskovitz-Eldor J
    Biotechnol. Bioeng., 2004;88(3):313-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4 (Based on 3 reviews)

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We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 applications: Western Blot, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Nestin Antibody MAB1259
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Very Good
 WB Human Anonymous 10/26/2015
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Western Blot Human Nestin Antibody MAB1259
Western Blot: Human Nestin Antibody [MAB1259]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 5% non-fat milk in TBST
Dilution: 1/1000
Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259
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Very Good
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259
Immunocytochemistry/Immunofluorescence: Human Nestin Antibody [MAB1259]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHuman cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100
Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259
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Very Good
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259
Immunocytochemistry/Immunofluorescence: Human Nestin Antibody [MAB1259]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHeLa cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.1% Triton X-100 in PBS
Dilution: 1/100

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