Human Neuregulin-1/NRG1 Antibody

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Product Details
Citations (10)
Supplemental Products

Human Neuregulin-1/NRG1 Antibody Summary

Species Reactivity
Detects human Neuregulin‑1/NRG1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with the EGF‑like domains of recombinant human (rh) NRG1-beta 1 (aa 176‑246) or rhNRG1-alpha (aa 177‑241). Therefore, this antibody recognizes an epitope located within aa 2‑176 segment N‑terminal to the EGF‑like domain. This segment is present in all NRG1‑ alpha, beta and gamma isoforms and in NDF43 and glial growth factor (GGF). It is absent from the NRG1 gene products GGF2 and SMDF.
Monoclonal Mouse IgG2B Clone # 147724
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Neuregulin‑1/NRG1
Accession # AAA58639
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Neuregulin‑1/NRG1 (Catalog # 5898-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-1/NRG1

The Neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  1. Buonanno, A., and G.D Fischbach (2001) Curr. Opin. Neurobiol. 11:287.
  2. Adlkofer, K. and C. Lai (2000) Glia 29:104.
  3. Garratt, A.N. et al., (2000) BioEssays 22:987.
Entrez Gene IDs
3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names
ARIA; GGF2; GGFglial growth factor; Heregulin-1; HGL; HGLneu differentiation factor; HRG; HRG1; HRG1-alpha; HRG1-beta 1; HRGAneuregulin 1 type IV beta 1a; MST131; MSTP131; NDFheregulin, alpha (45kD, ERBB2 p185-activator); neuregulin 1 type IV beta 3; neuregulin 1; Neuregulin1; Neuregulin-1; NRG1; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF

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Citations for Human Neuregulin-1/NRG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Adding of neurotensin to non-small cell lung cancer cells increases tyrosine phosphorylation of HER3
    Authors: TW Moody, I Ramos-Alva, RT Jensen
    Peptides, 2022;156(0):170858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
    Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
    Nat Commun, 2019;10(1):1365.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis
    Authors: T Rahim, P Becquart, ME Baeva, J Quandt
    J Neuroinflammation, 2018;15(1):270.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  4. BRD4 amplification facilitates an oncogenic gene expression program in high-grade serous ovarian cancer and confers sensitivity to BET inhibitors
    Authors: GW Rhyasen, Y Yao, J Zhang, A Dulak, L Castriotta, K Jacques, W Zhao, F Gharahdagh, MM Hattersley, PD Lyne, E Clark, M Zinda, SE Fawell, GB Mills, H Chen
    PLoS ONE, 2018;13(7):e0200826.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling
    Authors: DM Shi, LX Li, XY Bian, XJ Shi, LL Lu, HX Zhou, TJ Pan, J Zhou, J Fan, WZ Wu
    J. Exp. Clin. Cancer Res., 2018;37(1):294.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Dual effects of carbon monoxide on pericytes and neurogenesis in traumatic brain injury
    Nat Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Fibroblast-derived neuregulin 1 promotes compensatory ErbB3 receptor signaling in mutant BRAF melanoma.
    Authors: Capparelli C, Rosenbaum S, Berger A, Aplin A
    J Biol Chem, 2015;290(40):24267-77.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. BRCA1-IRIS inactivation sensitizes ovarian tumors to cisplatin.
    Authors: Paul B, Blanchard Z, Ridgway M, ElShamy W
    Oncogene, 2015;34(23):3036-52.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  9. Fractalkine/CX3CL1 enhances GABA synaptic activity at serotonin neurons in the rat dorsal raphe nucleus.
    Authors: Heinisch S, Kirby LG
    Neuroscience, 2009;164(3):1210-23.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. The NRG1 gene is frequently silenced by methylation in breast cancers and is a strong candidate for the 8p tumour suppressor gene.
    Authors: Chua YL, Ito Y, Pole JC, Newman S, Chin SF, Stein RC, Ellis IO, Caldas C, O'Hare MJ, Murrell A, Edwards PA
    Oncogene, 2009;28(46):4041-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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