Human Neuroplastin Isoform 1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Neuroplastin by Western Blot.
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Product Details
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Human Neuroplastin Isoform 1 Antibody Summary

Species Reactivity
Detects human Neuroplastin in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 55 isoform 1
Accession # Q9Y639.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neuroplastin antibody by Western Blot. View Larger

Detection of Human Neuroplastin by Western Blot. Western blot shows lysates of human brain (cerebellum, hypothalamus, and hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neuroplastin antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Neuroplastin in Human Brain. Neuroplastin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 1.7 µg/mL Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the dendrites of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroplastin

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52‑57 kDa member of the Ig-superfamily. It is widely expressed, and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32‑119 and 122‑213) and a 38 aa cytoplasmic region (aa 245‑282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N-terminal 116 aa Ig-like domain-containing insert after Asn30, and interacts homophilically. There is a third potential splice variant that shows an alternate start site at Met85 accompanied by a 23 aa substitution for aa 200‑282. Over aa 29‑229, human Neuroplastin is 91% aa identical to mouse Neuroplastin.

Entrez Gene IDs
27020 (Human); 20320 (Mouse); 56064 (Rat)
Alternate Names
AW554172; GP55; GP55Stromal cell-derived receptor 1; GP65SDR-1; GP66; MGC102805; Neuroplastin; np55; np65; NPTN; SDFR1; SDFR1DKFZp686L2477; SDR1; SDR1stromal cell derived factor receptor 1

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