Human NHA2/SLC9B2 APC-conjugated Antibody

FAB8404A has been discontinued. View all NHA2/SLC9B2 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG2B Clone # 896151
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2
    Met1-Val537
    Accession # NP_849155
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP was stained with either (A) Mouse Anti-Human NHA2/SLC9B2 APC‑conjugated Monoclonal Antibody (Catalog # FAB8404A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NHA2/SLC9B2
NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.
  • Long Name:
    Sodium/Hydrogen Cation Proton Antiporter 2
  • Entrez Gene IDs:
    133308 (Human); 7086 (Mouse); 679958 (Rat)
  • Alternate Names:
    FLJ23984; Mitochondrial Na(+)/H(+) exchanger NHA2; mitochondrial sodium/hydrogen exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; Na+/H+ exchanger domain containing 2; NHA2; NHA2NHE10; NHE domain-containing protein 2; NHE10; NHEDC2; SLC9B2; Sodium/hydrogen exchanger-like domain-containing protein 2
Related Research Areas

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