Human NHA2/SLC9B2 APC-conjugated Antibody

Name: Human NHA2/SLC9B2 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB8404A

Catalog #: FAB8404A Datasheet / COA / SDS

Discontinued Product

FAB8404A has been discontinued.

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Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry.

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Human NHA2/SLC9B2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Mouse IgG_2B Clone # 896151

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2
Met1-Val537
Accession # NP_849155

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP was stained with either (A) Mouse Anti-Human NHA2/SLC9B2 APC-conjugated Monoclonal Antibody (Catalog # FAB8404A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NHA2/SLC9B2

NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.

Long Name

Sodium/Hydrogen Cation Proton Antiporter 2

Entrez Gene IDs

133308 (Human); 7086 (Mouse); 679958 (Rat)

Alternate Names

FLJ23984; Mitochondrial Na(+)/H(+) exchanger NHA2; mitochondrial sodium/hydrogen exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; Na+/H+ exchanger domain containing 2; NHA2; NHA2NHE10; NHE domain-containing protein 2; NHE10; NHEDC2; SLC9B2; Sodium/hydrogen exchanger-like domain-containing protein 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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