Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody
Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of NKG2D/CD314 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB139N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.
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- NKG2D and its Ligands (2002) www.RnDSystems.com.
Citations for Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody
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Citations: Showing 1 - 2
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Transmissible cytotoxicity of multiple myeloma cells by cord blood-derived NK cells is mediated by vesicle trafficking.
Authors: Martin-Antonio B, Najjar A, Robinson S, Chew C, Li S, Yvon E, Thomas M, Mc Niece I, Orlowski R, Munoz-Pinedo C, Bueno C, Menendez P, Fernandez de Larrea C, Urbano-Ispizua A, Shpall E, Shah N
Cell Death Differ, 2015;22(1):96-107.
Sample Types: Whole Cells
Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
PLoS Pathog, 2015;11(3):e1004671.
Sample Types: Whole Cells
Applications: Flow Cytometry
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