Human NKG2D/CD314 Alexa Fluor® 700-conjugated Antibody

Clone 149810 was used by HLDA to establish CD designation.
(2 citations)   
  • Species Reactivity
  • Specificity
    Detects human NKG2D/CD314 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 149810
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKG2D/CD314 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB139N) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  • References:
    1. Li, P. et al. (2001) Nature Immunol. 2:443.
    2. Steinle, A. et al. (2001) Immunogenetics 53:279.
    3. Cosman, D. et al. (2001) Immunity 14:123.
    4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
    5. Wu, J. et al. (1999) Science 285:730.
    6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
    7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
    8. Groh, V. et al. (2001) Nature Immunol. 2:255.
    9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
    10. Diefenbach, A. et al. (2001) Nature 413:165.
    11. NKG2D and its Ligands (2002)
  • Long Name:
    Natural Killer G2D
  • Entrez Gene IDs:
    22914 (Human); 27007 (Mouse)
  • Alternate Names:
    CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
    Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
    PLoS Pathog, 2015;11(3):e1004671.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Transmissible cytotoxicity of multiple myeloma cells by cord blood-derived NK cells is mediated by vesicle trafficking.
    Authors: Martin-Antonio B, Najjar A, Robinson S, Chew C, Li S, Yvon E, Thomas M, Mc Niece I, Orlowski R, Munoz-Pinedo C, Bueno C, Menendez P, Fernandez de Larrea C, Urbano-Ispizua A, Shpall E, Shah N
    Cell Death Differ, 2015;22(1):96-107.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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