Human NKG2D/CD314 APC-conjugated Antibody

Clone 149810 was used by HLDA to establish CD designation.
(6 citations)   
  • Species Reactivity
  • Specificity
    Detects human NKG2D/CD314 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 149810
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human
NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 APC‑conjugated Monoclonal Antibody (Catalog # FAB139A) or (B) Mouse IgG1 APC Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  • References:
    1. Li, P. et al. (2001) Nature Immunol. 2:443.
    2. Steinle, A. et al. (2001) Immunogenetics 53:279.
    3. Cosman, D. et al. (2001) Immunity 14:123.
    4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
    5. Wu, J. et al. (1999) Science 285:730.
    6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
    7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
    8. Groh, V. et al. (2001) Nature Immunol. 2:255.
    9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
    10. Diefenbach, A. et al. (2001) Nature 413:165.
    11. NKG2D and its Ligands (2002)
  • Long Name:
    Natural Killer G2D
  • Entrez Gene IDs:
    22914 (Human); 27007 (Mouse)
  • Alternate Names:
    CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 6 of 6
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Sample Type
  1. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
    Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
    J Immunol, 2014;193(4):1654-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. TL1A induces TCR independent IL-6 and TNF-alpha production and growth of PLZF(+) leukocytes.
    Authors: Reichwald K, Jorgensen T, Tougaard P, Skov S
    PLoS ONE, 2014;9(1):e85793.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
    Authors: Markel G, Seidman R, Besser MJ
    PLoS ONE, 2009;4(5):e5597.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. CD27 defines phenotypically and functionally different human NK cell subsets.
    Authors: Vossen MT, Matmati M, Hertoghs KM, Baars PA, Gent MR, Leclercq G, Hamann J, Kuijpers TW, van Lier RA
    J. Immunol., 2008;180(6):3739-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells.
    Authors: Kim YJ, Han MK, Broxmeyer HE
    Blood, 2007;111(3):1378-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Phosphostim-activated gamma delta T cells kill autologous metastatic renal cell carcinoma.
    Authors: Viey E, Fromont G, Escudier B, Morel Y, Da Rocha S, Chouaib S, Caignard A
    J. Immunol., 2005;174(3):1338-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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