Human NKG2D/CD314 PE-conjugated Antibody

Clone 149810 was used by HLDA to establish CD designation.
(22 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NKG2D/CD314 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 149810
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKG2D/CD314 in Human PBMCs gated on CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 PE‑conjugated Monoclonal Antibody (Catalog # FAB139P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  • References:
    1. Li, P. et al. (2001) Nature Immunol. 2:443.
    2. Steinle, A. et al. (2001) Immunogenetics 53:279.
    3. Cosman, D. et al. (2001) Immunity 14:123.
    4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
    5. Wu, J. et al. (1999) Science 285:730.
    6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
    7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
    8. Groh, V. et al. (2001) Nature Immunol. 2:255.
    9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
    10. Diefenbach, A. et al. (2001) Nature 413:165.
    11. NKG2D and its Ligands (2002) www.RnDSystems.com.
  • Long Name:
    Natural Killer G2D
  • Entrez Gene IDs:
    22914 (Human); 27007 (Mouse)
  • Alternate Names:
    CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017;7(0):44544.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Interferon Alpha Induces Sustained Changes in NK Cell Responsiveness to Hepatitis B Viral Load Suppression In Vivo
    PLoS Pathog, 2016;12(8):e1005788.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Natural killer (NK) cells inhibit systemic metastasis of glioblastoma cells and have therapeutic effects against glioblastomas in the brain.
    Authors: Lee S, Kang W, Yoon Y, Jin J, Song H, Her J, Kang S, Hwang Y, Kang K, Joo K, Nam D
    BMC Cancer, 2015;15(1):1011.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.
    Authors: Picardi A, Mengarelli A, Marino M, Gallo E, Benevolo M, Pescarmona E, Cocco R, Fraioli R, Tremante E, Petti M, De Fabritiis P, Giacomini P
    J Exp Clin Cancer Res, 2015;34(0):98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
    Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
    PLoS ONE, 2015;10(5):e0125544.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. NKG2D-directed cytokine-activated killer lymphocyte therapy combined with gemcitabine for patients with chemoresistant metastatic solid tumors.
    Authors: Morisaki T, Hirano T, Koya N, Kiyota A, Tanaka H, Umebayashi M, Onishi H, Katano M
    Anticancer Res, 2014;34(8):4529-38.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Long-term effect on natural killer cells by interferon-alpha therapy on the outcomes of HCV infection.
    Authors: Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
    J Interferon Cytokine Res, 2014;34(5):366-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells.
    Authors: Molfetta R, Quatrini L, Capuano C, Gasparrini F, Zitti B, Zingoni A, Galandrini R, Santoni A, Paolini R
    Eur J Immunol, 2014;44(9):2761-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. An epithelial cell adhesion molecule- and CD3-bispecific antibody plus activated T-cells can eradicate chemoresistant cancer stem-like pancreatic carcinoma cells in vitro.
    Authors: Umebayashi M, Kiyota A, Koya N, Tanaka H, Onishi H, Katano M, Morisaki T
    Anticancer Res, 2014;34(8):4509-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012;7(11):e49135.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling.
    Authors: Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R
    Diabetes, 2011;60(3):857-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2011;134(2):113-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Combinatorial Cytotoxicity of Gemcitabine and Cytokine-activated Killer Cells in Hepatocellular Carcinoma via the NKG2D-MICA/B System.
    Authors: Morisaki T, Onishi H, Koya N
    Anticancer Res., 2011;31(7):2505-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behcet's disease patients and healthy controls.
    Authors: Clemente A, Cambra A, Munoz-Saa I, Crespi C, Pallares L, Juan A, Matamoros N, Julia MR
    Immunol. Lett., 2010;129(2):57-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Functional impairment in circulating and intrahepatic NK cells and relative mechanism in hepatocellular carcinoma patients.
    Authors: Cai L, Zhang Z, Zhou L, Wang H, Fu J, Zhang S, Shi M, Zhang H, Yang Y, Wu H, Tien P, Wang FS
    Clin. Immunol., 2008;129(3):428-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil.
    Authors: Teixeira-Carvalho A, Fujiwara RT, Stemmy EJ, Olive D, Damsker JM, Loukas A, Correa-Oliveira R, Constant SL, Bethony JM
    Infect. Immun., 2008;76(12):5810-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Endometrial NK cells are special immature cells that await pregnancy.
    Authors: Manaster I, Mizrahi S, Goldman-Wohl D, Sela HY, Stern-Ginossar N, Lankry D, Gruda R, Hurwitz A, Bdolah Y, Haimov-Kochman R, Yagel S, Mandelboim O
    J. Immunol., 2008;181(3):1869-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008;384(5):1143-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells.
    Authors: Kim YJ, Han MK, Broxmeyer HE
    Blood, 2007;111(3):1378-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  22. Phosphostim-activated gamma delta T cells kill autologous metastatic renal cell carcinoma.
    Authors: Viey E, Fromont G, Escudier B, Morel Y, Da Rocha S, Chouaib S, Caignard A
    J. Immunol., 2005;174(3):1338-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 22 Citations
Isotype Controls
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Flow Cytometry Mouse Lyse Buffer (10X)

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Flow Cytometry Human Lyse Buffer (10X)

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