Human NLRP2/NALP2 Antibody

Catalog # Availability Size / Price Qty
AF4684
AF4684-SP
Detection of Human NLRP2/NALP2 by Western Blot.
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Human NLRP2/NALP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NLRP2/NALP2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NLRP2/NALP2 antibody by Western Blot. View Larger

Detection of Human NLRP2/NALP2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NLRP2/NALP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4684) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP2/NALP2

NALP2 (NAcht Leucine-rich repeat Protein 2, also known as NLRP2 and PAN1) is a cytosolic, 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF-alpha production in response to LPS; via procaspase-1, it promotes proIL-1 beta cleavage and release. Human NALP2 is 1062 amino acids (aa) in length. It contains an N-terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa’s 109‑132, 133‑154 and 847‑1062, respectively; a fourth shows a 38 aa substitution for aa’s 1‑903. No close rodent counterparts have been reported.

Long Name
NLR Family, Pyrin Domain Containing 2
Entrez Gene IDs
55655 (Human); 232827 (Mouse)
Alternate Names
CLR19.9; FLJ20510; NACHT, leucine rich repeat and PYD containing 2; NACHT, LRR and PYD domains-containing protein 2; NALP2; NALP2NACHT, LRR and PYD containing protein 2; NBS1; NBS1PYRIN-containing APAF1-like protein 2; NLR family, pyrin domain containing 2; NLRP2; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 2; PAN1; PAN1Nucleotide-binding site protein 1; PYPAF2; PYPAF2PYRIN domain and NACHT domain-containing protein 1

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