Human Nox4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Nox4 by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products

Human Nox4 Antibody Summary

Species Reactivity
Detects human Nox4 in direct ELISAs and Western blots. 
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Nox4
Accession # Q9NPH5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Nox4 antibody by Western Blot. View Larger

Detection of Human Nox4 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Nox4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8158) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nox4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nox4

NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases.  NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling.  NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin.  Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress.  Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified.  Over aa 450-578, human NOX4 has 92% sequence identity with mouse NOX4, and 91% identity with rat NOX4.

Long Name
NADPH Oxidase 4
Entrez Gene IDs
50507 (Human); 50490 (Mouse); 85431 (Rat)
Alternate Names
EC 1.6.3; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX; KOX-1; KOX-1Renal NAD(P)H-oxidase; NADPH oxidase 4; Nox4; RENOX; RENOXEC 1.6.3.-

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Citations for Human Nox4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Modulation of H4K16Ac levels reduces pro-fibrotic gene expression and mitigates lung fibrosis in aged mice
    Authors: X Zhang, H Liu, JQ Zhou, S Krick, JW Barnes, VJ Thannickal, YY Sanders
    Theranostics, 2022;12(2):530-541.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Brd4-p300 inhibition down-regulates Nox4 and accelerates lung fibrosis resolution in aged mice
    Authors: YY Sanders, X Lyu, QJ Zhou, Z Xiang, D Stanford, S Bodduluri, SM Rowe, VJ Thannickal
    JCI Insight, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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