Human Nox4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Nox4 in direct ELISAs and Western blots. 
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Nox4
    Lys450-Ser578
    Accession # Q9NPH4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Nox4 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Nox4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8158) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nox4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nox4

NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases.  NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling.  NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin.  Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress.  Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified.  Over aa 450-578, human NOX4 has 92% sequence identity with mouse NOX4, and 91% identity with rat NOX4.

  • Long Name:
    NADPH Oxidase 4
  • Entrez Gene IDs:
    50507 (Human); 50490 (Mouse); 85431 (Rat)
  • Alternate Names:
    EC 1.6.3; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX; KOX-1; KOX-1Renal NAD(P)H-oxidase; NADPH oxidase 4; Nox4; RENOX; RENOXEC 1.6.3.-
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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