Human NrCAM Isoform 3 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2034
Product Details
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Human NrCAM Isoform 3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NrCAM Isoform 3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant human (rh) NCAM-1 and rhNCAM-L1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NrCAM isoform 3
Leu30-Asn600 (Ala526Pro)
Accession # Q14CA1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR)

Human NrCAM Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human NrCAM Antibody (Catalog # MAB20341)

Standard: Recombinant Human NrCAM Fc Chimera Protein, CF (Catalog # 2034-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NrCAM

NgCAM related cell adhesion molecule (NrCAM), also known as Bravo, belongs to the L1 family of cell adhesion molecules, which also include L1, neurofascin and close homolog of L1 (CHL-1) (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like domains in their extracellular domain. They also shared a conserved cytoplasmic region containing an ankyrin-binding site. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (1, 2). NrCam mediates homophilic adhesion as well as heterophilic adhesion with a number of neuronal adhesion molecules including contactin, TAG-1/contactin-2, neurofascin and receptor tyrosine phosphatase beta (RPTP beta ) (3‑5). NrCAM has been implicated in the axogenesis of multiple neuronal populations including sensory axons and commissural axons in the spinal cord of chick embryos. It also plays a role in cerebellar granule cell development, and is required in node of Ranvier formation (5, 6).

References
  1. Hortsch, M. (2000) Mol. Cell. Neurosci. 15:1.
  2. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.
  3. Mauro, V.P. et al. (1992) 119:191.
  4. Fustig, M. Sakurai, T. and M. Grumet (1999) Dev. Bio. 209:340.
  5. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.
  6. Custer, A.W. et al. (2003) J. Neuroscience 23:10032.
Long Name
Neuronal Cell Adhesion Molecule
Entrez Gene IDs
4897 (Human); 319504 (Mouse)
Alternate Names
Bravo; hBravo; KIAA0343Neuronal surface protein Bravo; MGC138845; MGC138846; neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule; ng-CAM-related; NrCAM; nr-CAM

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