Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.
Human NT-ProANP Quantikine ELISA Kit
R&D Systems | Catalog # DANP00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
0.146 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human NT-ProANP Quantikine ELISA Kit
The Quantikine® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human NT-ProANP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.71 | 6.04 | 11.1 | 1.81 | 5.95 | 11.5 |
| Standard Deviation | 0.069 | 0.224 | 0.583 | 0.129 | 0.426 | 0.842 |
| CV% | 4.0 | 3.7 | 5.3 | 7.1 | 7.2 | 7.3 |
Recovery for Human NT-ProANP Quantikine ELISA Kit
The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 87-114 |
| EDTA Plasma (n=4) | 102 | 84-120 |
| Heparin Plasma (n=4) | 98 | 84-111 |
| Serum (n=4) | 108 | 95-122 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human NT-ProANP Quantikine ELISA Kit
Human NT-ProANP ELISA Standard Curve
Endothelin Induces Expression of Atrial Natriuretic Peptide in iPSC-derived Cardiomyoctyes.
JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (ANP), which is known to be elevated during cardiac hypertrophy, was assessed using the Human NT-ProANP Quantikine® ELISA Kit (Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Atrial Natriuretic Peptide/ANP
Alternate Names
ANF, ANP, Cardionatrin, NPPA, PND
Gene Symbol
NPPA
Additional Atrial Natriuretic Peptide/ANP Products
Product Documents for Human NT-ProANP Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human NT-ProANP Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human NT-ProANP Quantikine ELISA Kit
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Protocols
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