Human NT-ProANP Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DANP00
Control Products Available
Human NT-ProANP ELISA Standard Curve
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human NT-ProANP Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.146 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human NT-ProANP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.71 6.04 11.1 1.81 5.95 11.5
Standard Deviation 0.069 0.224 0.583 0.129 0.426 0.842
CV% 4 3.7 5.3 7.1 7.2 7.3

Recovery

The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 87-114
EDTA Plasma (n=4) 102 84-120
Heparin Plasma (n=4) 98 84-111
Serum (n=4) 108 95-122

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human NT-ProANP ELISA Linearity

Data Examples

Human NT-ProANP ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Atrial Natriuretic Peptide/ANP

Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.

Entrez Gene IDs:
4878 (Human); 230899 (Mouse); 24602 (Rat)
Alternate Names:
ANF; ANP; ANPatriopeptin; ATFB6; atrial natriuretic factor; Atrial Natriuretic Peptide; Cardionatrin; CDD-ANF; natriuretic peptide A; natriuretic peptide precursor A; NPPA; PND; PNDcardionatrin; prepronatriodilatin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

ELISA Controls

Supplemental ELISA Products

Reviews for Human NT-ProANP Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human NT-ProANP Quantikine ELISA Kit and earn rewards!

Have you used Human NT-ProANP Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review