Human NT-ProANP Quantikine ELISA Kit Summary
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||104||87-114|
|EDTA Plasma (n=4)||102||84-120|
|Heparin Plasma (n=4)||98||84-111|
Human NT-ProANP ELISA Standard Curve
Preparation and Storage
Background: Atrial Natriuretic Peptide/ANP
Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.
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