Endothelin 1 (human, porcine)

Tocris Bioscience | Catalog # 1160

Potent vasoconstrictor peptide
Tocris Bioscience

Key Product Details

Description

Potent vasoconstrictor peptide

Alternative Names

ET-1
View all Non-selective Endothelin Receptor Agonists »

Product Description

Endothelin 1 (human, porcine) is a potent endogenous peptide agonist at ETA and ETB receptors. Produces strong vasoconstriction and pressor responses. Competitive binding assay shows that Endothelin 1 reversibly binds to receptors (IC50 values are 0.12 nM and 0.15 nM at ETB and ETA receptors respectively). Potency is dependent on blood vessel type (EC50 ranges from 2.72 nM - 17.4 nM). Activates PLC (EC50 = 0.3 nM), increases production of PGE2 (EC50 = 12 nM) and induces intracellular calcium release in human ciliary muscle cells. Also upregulates leptin mRNA expression and secretion in adipocytes (EC50 = 8.8 nM).

Scientific Data Images for Endothelin 1 (human, porcine)

cardiac hypertrophy assay

Pluripotent stem cell-derived cardiomyocytes secrete Pro-BNP in response to small molecule-induction of cardiac hypertrophy

Cardiomyocytes were treated with the vasoconstrictor, ET-1 (Endothelin-1; Catalog # 1160), or one of two L-type calcium channel inhibitors, Doxorubicin (Catalog # 2252) or Verapamil (Catalog # 0654). Levels of the cardiac hypertrophy marker, Pro-Brain Natriuretic Peptide (Pro-BNP), were detected via Luminex® bead-based multiplex assay (Catalog # LXSAH). A substantial increase in Pro-BNP was detected in the medium of cardiomyocytes treated with ET-1, a known inducer of cardiac hypertrophy. Conversely, Doxorubicin and Verapamil decreased Pro-BNP secretion from cardiomyocytes.

ET1 differentiated cardiomyocytes concentration dependent effect on Pro-ANP

Differentiated cardiomyocytes respond physiologically to Endothelin-1 induced hypertrophy

Cardiomyocytes differentiated from BG01V human embryonic stem cells were treated with Endothelin-1 (ET-1; Catalog # 1160) for 28 hours to induce cardiac hypertrophy. Levels of Pro-Atrial Natriuretic peptide (Pro-ANP) secretion, which is known to be elevated during cardiac hypertrophy, were assessed using ELISA (Catalog # DANP00). As expected, detection of Pro-ANP increased proportionally with the concentration of Endothelin-1 treatment.

western blot and ELISA ANP expression in cardiomyocytes derived from iPSCs

Endothelin Induces Expression of Atrial Natriuretic Peptide in Cardiomyocytes Derived from iPSCs

JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (ET-1; Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (Pro-ANP), which is known to be elevated during cardiac hypertrophy, was assessed using (A) Western Blot and (B) ELISA (Human NTProANP Quantikine® ELISA; Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.

Product Specifications for Endothelin 1 (human, porcine)

Molecular Weight

2492.00

Formula

C109H159O32N25S5

Storage

Store at -20°C

Purity

≥95% (HPLC)

CAS Number

117399-94-7

PubChem ID

16133807

InChI Key

ZUBDGKVDJUIMQQ-ZTNLKOGPSA-N

SMILES

[H][C@](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC1=CNC=N1)NC(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@]2([H])CSSC[C@]([H])(NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC2=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC2=CC=C(O)C=C2)C(=O)N[C@H](CC2=CC=CC=C2)C(=O)N1)([C@@H](C)CC)C(=O)N[C@@]([H])([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 1mg/ml in water

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 33 publications citing the usage of this product.

Product Documents for Endothelin 1 (human, porcine)

Certificate of Analysis

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Product Specific Notices for Endothelin 1 (human, porcine)

For research use only

Citations for Endothelin 1 (human, porcine)

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