Detection of NTAL in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Sheep Anti-Human NTAL Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4066, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human NTAL by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NTAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NTAL at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Non-T cell activation linker (NTAL), also known as linker for activation of B cells (LAB), is a transmembrane adaptor protein involved in immunoreceptor signaling. NTAL is expressed in lipid raft microdomains of B cells, mast cells, monocytes and NK cells. Rapid tyrosine phosphorylation of NTAL occurs upon BCR aggregation in B cells, Fc epsilon RI aggregation and Kit activation in mast cells, and Fc gamma RI aggregation in monocytes. Phosphorylated NTAL recruits signaling molecules such as Grb2, Gab1, and c-Cbl into receptor-signaling complexes. Defects in the NTAL gene may cause Williams-Beuren syndrome, a rare genetic disorder characterized by mild mental retardation, and abnormalities in the cardiovascular and musculo-skeletal systems.
Non-T Cell Activation Linker
Entrez Gene IDs:
7462 (Human); 56743 (Mouse); 317676 (Rat)
LAB; LABWilliams-Beuren syndrome chromosomal region 15 protein; Linker for activation of B-cells; linker for activation of T cells family, member 2; linker for activation of T-cells family member 2; Membrane-associated adapter molecule; Non-T-cell activation linker; NTAL; NTALWilliams-Beuren syndrome chromosomal region 5 protein; WBS15; WBSCR15; WBSCR5; WBSCR5HSPC046; Williams-Beuren syndrome chromosome region 5; WSCR5
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