Human OSMR beta Alexa Fluor® 488-conjugated Antibody

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FAB4389G-100UG
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Human OSMR beta Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human OSM R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse OSM R beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 469221
Immunogen
Mouse myeloma cell line NS0-derived recombinant human OSM R beta
Glu28-Ser739
Accession # Q99650
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: OSMR beta

OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).

References
  1. Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. 
  2. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. 
  3. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. 
  4. Gearing, D.P. et al. (1992) Science 255:1434. 
  5. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. 
  6. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.  
  7. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. 
  8. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
  9. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
  10. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
  11. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
  12. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
  13. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
  14. Tanaka, M. et al. (2003) Blood 102:3154.
  15. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.
Long Name
Oncostatin M Receptor beta
Entrez Gene IDs
9180 (Human); 18414 (Mouse); 310132 (Rat)
Alternate Names
IL-31 receptor subunit beta; IL-31R subunit beta; IL-31RB; IL-31R-beta; Interleukin-31 receptor subunit beta; MGC150627; MGC75127; oncostatin M receptor; oncostatin-M specific receptor beta subunit; oncostatin-M-specific receptor subunit beta; OSM R beta; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1

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