Human Osteoactivin/GPNMB Antibody
Human Osteoactivin/GPNMB Antibody Summary
Lys23-Asn486
Accession # NP_002501
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Applications
Human Osteoactivin/GPNMB Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Osteoactivin/GPNMB by Western Blot. Western blot shows lysates of U-118-MG human glioblastoma/astrocytoma cell line and T98G human glioblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Osteoactivin/GPNMB at approximately 95 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Osteoactivin/GPNMB in Human Liver. Osteoactivin/GPNMB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoactivin/GPNMB
Osteoactivin (also GPNMB and DC-HIL) is a variably glycosylated 75 - 125 kDa member of the NMB/pMEL-17 family of molecules. It is found in multiple subcellular sites, but is most often associated with the endosomal/lysosomal compartment (1-3). Human Osteoactivin is a 560 amino acid (aa) type I transmembrane protein. Its precursor contains a 21 aa signal sequence, a 465 aa luminal/extracellular domain, a 21 aa transmembrane segment and a 53 aa cytoplasmic tail (4, 5). The luminal region contains an N-terminal heparin-binding motif (aa 23-26), multiple glycosylation sites, an RGD motif (aa 64-66) and an 88 aa PKD domain (aa 240-327). The intracellular tail has an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif (4, 5). The extracellular/luminal region shares 74% and 77% aa identity with the equivalent regions in mouse and canine, respectively. Multiple isoforms would appear to exist. There is one alternate splice form known that shows a 12 aa insert between aa 339-340 (6). An addtional 206 aa isoform shows a mutation at position # 181 that results in a 26 aa substitution for the C-terminal 380 amino acids (7, 8). This has the potential to be soluble and may represent a counterpart to a secreted isoform of rat Osteoactivin (9). Cells known to express Osteoactivin include macrophages/Kupffer cells, fibroblasts, osteoblasts, myeloid dendritic cells, retinal pigment epithelial cells and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (3-5, 10-12). In mice, Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of endothelial cells and may also interact with integrins (13). It also appears to act as an inflammatory suppressor gene, as its expression downregulates the macrophage inflammatory response by inhibiting IL-6 and IL-12 p40 production (3).
- Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
- Safadi, F.F. et al. (2002) J. Cell. Biochem. 84:12.
- Ripoll, V.M. et al. (2007) J. Immunol. 178:6557.
- Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
- Weterman, M.A.J. et al. (1995) Int. J. Cancer 60:73.
- Kuan, C-T. et al. (2006) Clin. Cancer Res. 12:1970.
- Lennerz, V. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16013.
- Genbank Accession # AAH11595.
- Abdelmagid, S.M. et al. (2007) J. Cell. Physiol. 210:26.
- Haralanova-Ilieva, B. et al. (2005) J. Hepatol. 42:565.
- Ahn, J.H. et al. (2002) Blood 100:1742.
- Anderson, M.G. et al. (2002) Nat. Genet. 30:81.
- Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
Product Datasheets
Citations for Human Osteoactivin/GPNMB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A novel missense mutation in the folliculin gene associated with the renal tumor-only phenotype of Birt-Hogg-Dub� syndrome
Authors: T Sano, T Fukui, N Makita, K Shimizu, J Kono, K Masui, T Sato, T Goto, A Sawada, M Fujimoto, F Kojima, M Torishima, T Wada, M Furuya, O Ogawa, T Kobayashi, S Akamatsu
Cancer genetics, 2022;266(0):28-32.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns
Authors: D Netanely, S Leibou, R Parikh, N Stern, H Vaknine, R Brenner, S Amar, RH Factor, T Perluk, J Frand, E Nizri, D Hershkovit, V Zemser-Wer, C Levy, R Shamir
Oncogene, 2021;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Amyloidosis cutis dyschromica cases caused by GPNMB mutations with different inheritance patterns
Authors: W Qin, H Wang, W Zhong, J Bai, J Qiao, Z Lin
Journal of dermatological science, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
Acta Neuropathol Commun, 2020;8(1):163.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Clinical and Molecular Characterization of Microphthalmia-Associated Transcription Factor (MITF)-Related Renal Cell Carcinoma
Authors: M Lang, CD Vocke, CJ Ricketts, AR Metwalli, MW Ball, LS Schmidt, WM Linehan
Urology, 2020;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
GPNMB is expressed in human epidermal keratinocytes but disappears in the vitiligo lesional skin
Authors: KB Biswas, A Takahashi, Y Mizutani, S Takayama, A Ishitsuka, L Yang, F Yang, A Iddamalgod, I Katayama, S Inoue
Sci Rep, 2020;10(1):4930.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of the kringle-like domain in glycoprotein NMB for its tumorigenic potential
Authors: R Xie, Y Okita, Y Ichikawa, MA Fikry, KT Huynh Dam, STP Tran, M Kato
Cancer Sci., 2019;110(7):2237-2246.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TFE3 Xp11.2 translocation renal cell carcinoma mouse model reveals novel therapeutic targets and identifies GPNMB as a diagnostic marker for human disease
Authors: M Baba, M Furuya, T Motoshima, M Lang, S Funasaki, W Ma, HW Sun, H Hasumi, Y Huang, I Kato, T Kadomatsu, Y Satou, N Morris, BO Karim, L Ileva, JD Kalen, LA Wilan Kris, Y Hasumi, A Sugiyama, R Kurahashi, K Nishimoto, M Oyama, Y Nagashima, N Kuroda, K Araki, M Eto, M Yao, T Kamba, T Suda, Y Oike, LS Schmidt, WM Linehan
Mol. Cancer Res., 2019;0(0):.
Species: Human
Sample Types: Paraffin-Embedded Tissue
Applications: IHC -
GPNMB methylation: a new marker of potentially carcinogenic colon lesions
Authors: H Ashktorab, H Rahi, M Nouraie, B Shokrani, E Lee, T Haydari, AO Laiyemo, P Siegel, H Brim
BMC Cancer, 2018;18(1):1068.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Direct molecular dissection of tumor parenchyma from tumor stroma in tumor xenograft using mass spectrometry-based glycoproteomics
Authors: X Ye, BT Luke, BR Wei, JA Kaczmarczy, J Loncarek, JE Dwyer, DJ Johann, RG Saul, DV Nissley, F McCormick, GR Whiteley, J Blonder
Oncotarget, 2018;9(41):26431-26452.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC-P -
Glycoprotein nmb is exposed on the surface of dormant breast cancer cells and induces stem cell-like properties
Authors: C Chen, Y Okita, Y Watanabe, F Abe, MA Fikry, Y Ichikawa, H Suzuki, A Shibuya, M Kato
Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: FACS -
Clinical Significance of Glycoprotein Non-metastatic B and Its Association with EGFR/HER2 in Gastrointestinal Cancer
Authors: JY Tajima, M Futamura, S Gaowa, R Mori, T Tanahashi, Y Tanaka, N Matsuhashi, T Takahashi, K Yamaguchi, T Miyazaki, K Yoshida
J Cancer, 2018;9(2):358-366.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exploratory study on the effect of osteoactivin on muscle regeneration in a rat volumetric muscle loss model
Authors: J Ma, AR Baker, A Calabro, KA Derwin
PLoS ONE, 2017;12(4):e0175853.
Species: N/A
Sample Types: Gel Constructs
Applications: IHC-Fr -
GPNMB ameliorates mutant TDP-43-induced motor neuron cell death
Authors: Yuki Nagahara
J. Neurosci. Res, 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Identification of a biomarker in cerebrospinal fluid for neuronopathic forms of Gaucher disease.
Authors: Zigdon, Hila, Savidor, Alon, Levin, Yishai, Meshcheriakova, Anna, Schiffmann, Raphael, Futerman, Anthony
PLoS ONE, 2015;10(3):e0120194.
Species: Human
Sample Types: CSF
Applications: Western Blot -
GPNMB/OA protein increases the invasiveness of human metastatic prostate cancer cell lines DU145 and PC3 through MMP-2 and MMP-9 activity.
Authors: Fiorentini C, Bodei S, Bedussi F, Fragni M, Bonini S, Simeone C, Zani D, Berruti A, Missale C, Memo M, Spano P, Sigala S
Exp Cell Res, 2014;323(1):100-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hematopoietic growth factor inducible neurokinin-1 (Gpnmb/Osteoactivin) is a biomarker of progressive renal injury across species.
Authors: Patel-Chamberlin M, Wang Y, Satirapoj B
Kidney Int., 2011;79(10):1138-48.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Glycoprotein nonmetastatic B is an independent prognostic indicator of recurrence and a novel therapeutic target in breast cancer.
Authors: Rose AA, Grosset AA, Dong Z, Russo C, Macdonald PA, Bertos NR, St-Pierre Y, Simantov R, Hallett M, Park M, Gaboury L, Siegel PM
Clin. Cancer Res., 2010;16(7):2147-56.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inactivation of the FLCN tumor suppressor gene induces TFE3 transcriptional activity by increasing its nuclear localization.
Authors: Hong SB, Oh H, Valera VA, Baba M, Schmidt LS, Linehan WM
PLoS ONE, 2010;5(12):e15793.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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