Human Osteopontin/OPN Antibody

Catalog # Availability Size / Price Qty
MAB1433
MAB1433-SP
Osteopontin/OPN in Human Breast Cancer Tissue.
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Product Details
Citations (5)
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Human Osteopontin/OPN Antibody Summary

Species Reactivity
Human
Specificity
Detects human Osteopontin in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 190312
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Osteopontin
Ile17-Asn300
Accession # NP_000573.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Osteopontin/OPN antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Osteopontin/OPN in Human Breast Cancer Tissue. Osteopontin/OPN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB1433) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta1(early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin binding ligand N-linked glycoprotein) family of noncollagenous matricellular proteins (1‑3). Human OPN is synthesized as a 317 aminoacid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1‑4). Depending on tissue-specific modification by O- and N‑glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45‑75 kDa (5, 6). The central region of OPN contains RGD and nonRGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6‑8). OPN can also be cleaved by MMP3, 7, 9, and 12 within the SVVYGLR motif and at sites closer to the C‑terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

References
  1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Young, M.F. et al. (1990) Genomics 7:491.
  4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
  5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
  6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
  7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
  8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  9. Takafuji, V. et al. (2007) Oncogene 26:6361.
  10. Kiefer, F.W. et al. (2010) Diabetes 59:935.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

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Citations for Human Osteopontin/OPN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. AntagomiR-29b inhibits vascular and valvular calcification and improves heart function in rats
    Authors: M Fang, K Liu, X Li, Y Wang, W Li, B Li
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. AntagomiR-29b inhibits vascular and valvular calcification and improves heart function in rats
    Authors: M Fang, K Liu, X Li, Y Wang, W Li, B Li
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Osteopontin expression and relation to disease severity in human asthma.
    Authors: Samitas K, Zervas E, Vittorakis S, Semitekolou M, Alissafi T, Bossios A, Gogos H, Economidou E, Lotvall J, Xanthou G, Panoutsakopoulou V, Gaga M
    Eur. Respir. J., 2011;37(2):331-41.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. The effect of human osteoblasts on proliferation and neo-vessel formation of human umbilical vein endothelial cells in a long-term 3D co-culture on polyurethane scaffolds.
    Authors: Hofmann A, Ritz U, Verrier S, Eglin D, Alini M, Fuchs S, Kirkpatrick CJ, Rommens PM
    Biomaterials, 2008;29(31):4217-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
    Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
    Nat. Med., 2007;13(5):570-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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