Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 190312
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Osteopontin
Ile17-Asn300
Accession # NP_000573.1

Specificity

Detects human Osteopontin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Osteopontin/OPN Antibody

Osteopontin/OPN antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Osteopontin/OPN in Human Breast Cancer Tissue.

Osteopontin/OPN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB1433) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Osteopontin/OPN by Immunocytochemistry/ Immunofluorescence

Detection of Osteopontin/OPN by Immunocytochemistry/ Immunofluorescence

SPP1 and OPN expression in BAL cells. (A) SSP1 relative mRNA levels in IPF and N-IPF relative to healthy (Brown-Forsythe Anova, with individual comparisons), (B) Violin plots of mean OPN expression/cell per disease group (Kruskal-Wallis test and individual comparisons) and (C) Typical Images of BAL cytospins stained with anti-human OPN antibody and ToPro-633 nuclear stain in (i) healthy, (ii) IPF and (iii) N-IPF samples (*p < 0.05, **P < 0.01, ****p < 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34867934), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Osteopontin/OPN Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Reviewed Applications

Read 2 reviews rated 4 using MAB1433 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta1(early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin binding ligand N-linked glycoprotein) family of noncollagenous matricellular proteins (1‑3). Human OPN is synthesized as a 317 aminoacid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1‑4). Depending on tissue-specific modification by O- and N‑glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45‑75 kDa (5, 6). The central region of OPN contains RGD and nonRGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6‑8). OPN can also be cleaved by MMP3, 7, 9, and 12 within the SVVYGLR motif and at sites closer to the C‑terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

References

  1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Young, M.F. et al. (1990) Genomics 7:491.
  4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
  5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
  6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
  7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
  8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  9. Takafuji, V. et al. (2007) Oncogene 26:6361.
  10. Kiefer, F.W. et al. (2010) Diabetes 59:935.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Entrez Gene IDs

6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)

Gene Symbol

SPP1

UniProt

NP_000573.1

Additional Osteopontin/OPN Products

Product Documents for Human Osteopontin/OPN Antibody

Certificate of Analysis

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Product Specific Notices for Human Osteopontin/OPN Antibody

For research use only

Citations for Human Osteopontin/OPN Antibody

Customer Reviews for Human Osteopontin/OPN Antibody (2)

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2 Customer Ratings
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  • Human Osteopontin/OPN Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 09/27/2023
    Human Osteopontin/OPN Antibody MAB1433
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20562127
    Species: Human
    Verified Customer | Posted 02/10/2015

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