P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
Discontinued Product
BBE6 has been discontinued.
An alternative/replacement product is available:
DPSE00. View all P-Selectin/CD62P products.
Key Product Details
Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.5 ng/mL
Assay Range
0.82-45.94 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
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Product Summary for Human P-Selectin/CD62P ELISA Kit
The Human sP-Selectin Immunoassay is a 1.25 hour solid phase ELISA that measures sP-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human sP-Selectin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant sP-Selectin. Results obtained using natural human sP-Selectin showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sP-Selectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human sP-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 10 | 10 | 10 | 18 | 18 | 18 |
| Mean (pg/mL) | 84 | 430 | 668 | 94 | 451 | 730 |
| Standard Deviation | 4.28 | 21.1 | 37.4 | 9.31 | 39.7 | 57.7 |
| CV% | 5.1 | 4.9 | 5.6 | 9.9 | 8.8 | 7.9 |
Recovery for Human P-Selectin/CD62P ELISA Kit
The recovery of sP-Selectin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 105 | 98-115 |
| Citrate Plasma (n=7) | 98 | 82-111 |
| EDTA Plasma (n=7) | 94 | 78-107 |
| Heparin Plasma (n=7) | 100 | 77-114 |
| Serum (n=7) | 101 | 84-119 |
Linearity
To assess the linearity of the assay, natural or spiked samples of each type were diluted in Sample Diluent and assayed in three batches of kits.
Scientific Data Images for Human P-Selectin/CD62P ELISA Kit
Human P-Selectin/CD62P Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: P-Selectin/CD62P
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
Additional P-Selectin/CD62P Products
Product Documents for Human P-Selectin/CD62P ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human P-Selectin/CD62P ELISA Kit
For research use only
Citations for Human P-Selectin/CD62P ELISA Kit
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Protocols
View specific protocols for Human P-Selectin/CD62P ELISA Kit (BBE6):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, samples, and Control as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Standard, Control, or sample to each well.
- Add 100 µL of Conjugate to each well. Tap the plate gently to mix. Cover with a plate sealer, and incubate at room temperature for 1 hour.
- Aspirate or decant each well and wash, repeating the process twice for a total of 3 washes.
- Add 100 µL of Substrate to each well. Cover with a new plate sealer, and incubate at room temperature for 15 minutes.
- Add 100 µL Stop Solution to each well. The Stop Solution should be added to the wells in the same order as the Substrate. Read at 450 nm within 30 minutes. Set wavelength correction to 620 nm or 650 nm.
100 µL Standard, Control, or sample
100 µL Conjugate
100 µL Substrate
100 µL Stop Solution
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