Intracellular Staining by Flow Cytometry
|Detection of p53 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line treated (filled histogram) with camptothecin (CPT) or untreated (open histogram) was stained with Mouse Anti-Human p53 APC‑conjugated Monoclonal Antibody (Catalog # IC13551A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
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