Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 344222
View all PAR2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085

Specificity

Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PAR2 APC‑conjugated Antibody

Detection of PAR2 antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

Detection of PAR2 in HT-29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 APC-conjugated Monoclonal Antibody (Catalog # FAB3949A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PAR2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HT-29 human colon adenocarcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PAR2

Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.

Long Name

Protease-Activated Receptor 2

Alternate Names

CFIIRL1, F2RL1, GPCR11

Entrez Gene IDs

2150 (Human); 14063 (Mouse); 116677 (Rat)

Gene Symbol

F2RL1

UniProt

P55085

Additional PAR2 Products

Product Documents for Human PAR2 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PAR2 APC‑conjugated Antibody

For research use only

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