Detection of PAR2 in HT‑29 Human Cell Line by Flow Cytometry.
HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 -
Structural insight into allosteric modulation of protease-activated receptor 2
Authors: RKY Cheng, C Fiez-Vanda, O Schlenker, K Edman, B Aggeler, DG Brown, GA Brown, RM Cooke, CE Dumelin, AS Doré, S Geschwindn, C Grebner, NO Hermansson, A Jazayeri, P Johansson, L Leong, R Prihandoko, M Rappas, H Soutter, A Snijder, L Sundström, B Tehan, P Thornton, D Troast, G Wiggin, A Zhukov, FH Marshall, N Dekker
Sample Type: Whole Cells
Application: Binding Model
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