Human Pax6 PE-conjugated Antibody Summary
Accession # P26367
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Pax6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Pax6 PE-conjugated Affinity-Purified Polyclonal Antibody (Catalog # IC8150P, filled histogram) or Sheep IgG PE-conjugated control Antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Pax6 (paired box 6; also Oculorhombin) is a 48-50 kDa member of the paired homeobox family of transcription factors. It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells. Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2. Human Pax6 is 422 amino acids (aa) in length. It contains an N-terminal paired box DNA-binding domain (aa 4-130), a Gly-rich central region (aa 131-209), a homeodomain (aa 213-269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279-422). Phosphorylation of the C-terminal domain at Thr281/304/373 promotes Pax6 activity. Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22-26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C-terminal DNA binding site. Human and mouse Pax6 are absolutely identical in aa sequence.
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