Human Pax6 Antibody

Catalog # Availability Size / Price Qty
AF8150
AF8150-SP
Detection of Human and Rat Pax6 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Human Pax6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pax6 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax1, rhPax2, rhPax3, rhPax4, rhPax5, and rhPax7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pax6
Met1-Arg272
Accession # P26367
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Rat Pax6 by Western Blot. View Larger

Detection of Human and Rat Pax6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and rat cortical stem cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pax6 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Pax6 in SA01 Human Embryonic Stem Cells. View Larger

Pax6 in SA01 Human Embryonic Stem Cells. Immersion fixed SA01 human embryonic stem cells were differentiated for 6 days with Recombinant Human Noggin (Catalog # 6057‑NG) and SB431542 (upper panel) or differentiated for 6 days with Recombinant Human BMP-4 (negative control, lower panel; Catalog # 314-BP). Pax6 was detected using Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) at 5 µg/mL. Cells were stained using an Alexa Fluor 488-conjugated Anti-Sheep IgG Secondary Antibody (green) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Images courtesy of Dr. Ron McKay, Leiber Institute for Brain Development, Baltimore, Maryland, USA.

Intracellular Staining by Flow Cytometry Detection of  Pax6 in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of Pax6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Pax6 Affinity-Purified Polyclonal Antibody (Catalog # AF8150, filled histogram) or Sheep IgG control Antibody (Catalog # 5-001-A, open histogram) followed by anti-Sheep IgG PE-conjugated Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human Pax6 by Simple WesternTM. View Larger

Detection of Human Pax6 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Pax6 at approximately 59 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48‑50 kDa member of the paired homeobox family of transcription factors.  It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells.  Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2.  Human Pax6 is 422 amino acids (aa) in length.  It contains an N-terminal paired box DNA-binding domain (aa 4‑130), a Gly-rich central region (aa 131‑209), a homeodomain (aa 213‑269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279‑422).  Phosphorylation of the C‑terminal domain at Thr281/304/373 promotes Pax6 activity.  Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22‑26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C‑terminal DNA binding site.  Human and mouse Pax6 are absolutely identical in aa sequence.

Long Name
Paired Box Gene 6
Entrez Gene IDs
5080 (Human); 18508 (Mouse); 25509 (Rat)
Alternate Names
keratitis); MGC17209; Oculorhombin; paired box 6; paired box protein Pax-6; Pax6

Product Datasheets

Citations for Human Pax6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
    Authors: X Gao, JJ Yourick, RL Sprando
    Stem Cell Res, 2018;31(0):193-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. TFAP2C regulates transcription in human naive pluripotency by opening enhancers
    Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
    Nat. Cell Biol., 2018;20(5):553-564.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Chronic Exposure to Palmitate Impairs Insulin Signaling in an Intestinal L-cell Line: A Possible Shift from GLP-1 to Glucagon Production
    Authors: A Filippello, F Urbano, S Di Mauro, A Scamporrin, A Di Pino, R Scicali, AM Rabuazzo, F Purrello, S Piro
    Int J Mol Sci, 2018;19(12):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Reviews for Human Pax6 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human Pax6 Antibody
By Anonymous on 09/26/2018
Application: ICC/IF Sample Tested: Neural progenitor cells Species: Human

Pax6 was detected human embryonic stem cells-derived neural stem cells using Sheep Anti-Human PAX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) at 1 µg/mL for overnight at 4°C. Cells were stained using the Donkey anti-sheep IgG (H+L) Cross-Adsorbed, Alexa Fluor® 568.


Human Pax6 Antibody
By Anonymous on 11/02/2017
Application: IHC Sample Tested: Cerebellum tissue Species: Mouse

Works fine at 1/1000 dilution. O.N. incubation at 4C.


Human Pax6 Antibody
By Anonymous on 07/13/2016
Application: IHC Sample Tested: Cerebellum tissue Species: Mouse