Human Pax6 Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48‑50 kDa member of the paired homeobox family of transcription factors.  It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells.  Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2.  Human Pax6 is 422 amino acids (aa) in length.  It contains an N-terminal paired box DNA-binding domain (aa 4‑130), a Gly-rich central region (aa 131‑209), a homeodomain (aa 213‑269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279‑422).  Phosphorylation of the C‑terminal domain at Thr281/304/373 promotes Pax6 activity.  Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22‑26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C‑terminal DNA binding site.  Human and mouse Pax6 are absolutely identical in aa sequence.

Product Datasheets

Related Research Areas

• Adult Neurogenesis
• Early Ectodermal Lineage Markers
• Hepatic and Pancreatic Progenitor Cell Transcription Factors
• Neural Progenitor Cell Markers
• Neural Stem Cell Markers
• Neural Stem Cell Transcription Factors
• Paired Box Transcription Factors
• Pancreatic Endoderm
• Pancreatic Endoderm Cell Markers
• Pluripotent Stem Cell Transcription Factors
• Retinal Stem Cell Markers
• Transcription Factors in the Akt Pathway