Human PD-1 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Human PD‑1 by Western Blot.
1 Image
Product Details
Citations (5)
Supplemental Products
Reviews (2)

Human PD-1 Antibody Summary

Species Reactivity
Detects human PD-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD28, rhCTLA4, rhICOS, or recombinant mouse PD-1 is observed.
Monoclonal Mouse IgG2B Clone # 192106
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human PD-1
Accession # Q8IX89
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PD-1 antibody by Western Blot. View Larger

Detection of Human PD‑1 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF Membrane was probed with 1 µg/mL of Human PD-1 Monoclonal Antibody (Catalog # MAB1086) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PD-1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3). Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both PD‑L1 and PD‑L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2). The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V‑type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
  4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

Product Datasheets

You must select a language.


Citations for Human PD-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Evaluation of the immune checkpoint factors in idiopathic membranous nephropathy
    Authors: R Motavalli, M Hosseini, MS Soltani-Za, A Karimi, M Sadeghi, S Dolati, M Yousefi, J Etemadi
    Molecular and cellular probes, 2023-04-21;69(0):101914.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement
    Authors: R Banga, C Rebecchini, FA Procopio, A Noto, O Munoz, K Ioannidou, C Fenwick, K Ohmiti, M Cavassini, JM Corpataux, L de Leval, G Pantaleo, M Perreau
    PLoS Pathog., 2019-07-22;15(7):e1007918.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014-12-05;9(12):e113396.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  4. Splenic proliferative lymphoid nodules distinct from germinal centers are sites of autoantigen stimulation in immune thrombocytopenia.
    Authors: Daridon C, Loddenkemper C, Spieckermann S, Kuhl A, Salama A, Burmester G, Lipsky P, Dorner T
    Blood, 2012-09-06;120(25):5021-31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
    Authors: Sakthivel P, Ramanujam R, Wang XB, Pirskanen R, Lefvert AK
    J. Neuroimmunol., 2007-11-26;193(1):149-55.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PD-1 Antibody

Average Rating: 4 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human PD-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human PD-1 Antibody
By Anonymous on 08/28/2023
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

Lentivirus was used to infect 293T cells with either control or PD-1 overexpression. Following that, a PVDF membrane was employed and probed under reducing conditions using a 1 µm/ml Human PD-1 Antibody. A distinct band corresponding to PD-1 was identified at around 35 kDa.

Human PD-1 Antibody
By Qing Xu on 07/02/2018
Application: WB Sample Tested: Cancer cell lysates,HEK293 human embryonic kidney cell line Species: Human

293T cells were infected with letivirus carrying human PD-1 or control for 72h. Total cell lysates were collected and subject to Western Blot. HeLa protein sample was used as a PD-1 positive control. Membrane was probed with 1 µg/mL of Human PD‑1 Monoclonal Antibody (Catalog # MAB1086). PD‑1 band was detected at approximately 33 kDa