Human PDGF-BB Antibody

Catalog # Availability Size / Price Qty
AF-220-NA
AF-220-SP
Human PDGF-BB Antibody in Data
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Product Details
Citations (14)
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Human PDGF-BB Antibody Summary

Species Reactivity
Human
Specificity
Detects PDGF-BB in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF-BB (Catalog # 220-BB)
Neutralization
Measured by its ability to neutralize PDGF‑BB-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF-BB Antibody. View Larger

Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF-BB Antibody. Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-BB (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-220-NA). The ND50is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name
Platelet-derived Growth Factor BB
Entrez Gene IDs
5155 (Human); 24628 (Rat)
Alternate Names
PDGFBB; PDGF-BB

Product Datasheets

Citations for Human PDGF-BB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice
    Authors: J Huang, H Yin, SS Rao, PL Xie, X Cao, T Rao, SY Liu, ZX Wang, J Cao, Y Hu, Y Zhang, J Luo, YJ Tan, ZZ Liu, B Wu, XK Hu, TH Chen, CY Chen, H Xie
    Theranostics, 2018;8(9):2435-2446.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
    Authors: Koji Ueno
    Sci Rep, 2016;6(0):28538.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. VEGF-C regulates lymphangiogenesis and capillary stability by regulation of PDGF-B.
    Authors: Onimaru M, Yonemitsu Y, Fujii T, Tanii M, Nakano T, Nakagawa K, Kohno R, Hasegawa M, Nishikawa S, Sueishi K
    Am. J. Physiol. Heart Circ. Physiol., 2009;297(5):H1685-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model.
    Authors: Miyake T, Aoki M, Morishita R
    Cardiovasc. Res., 2008;79(4):706-14.
    Species: Rabbit
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008;68(7):2085-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
    Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
    Exp. Cell Res., 2008;314(11):2176-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity.
    Authors: Mahadevan VS, Campbell M, McKeown PP, Bayraktutan U
    Cardiovasc. Res., 2006;72(1):60-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
    Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
    Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005;20(4):790-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
    Authors: Inkinen K, Wolff H, Lindroos P, Ahonen J
    Connect. Tissue Res., 2003;44(1):19-29.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1.
    Authors: Zhou W, Park I, Pins M, Kozlowski JM, Jovanovic B, Zhang J, Lee C, Ilio K
    Endocrinology, 2003;144(10):4280-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
    Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
    Stem Cells, 0;34(4):1112-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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