Human PDGF-BB Quantikine ELISA Kit

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Human PDGF-BB ELISA Cell Culture Supernate Standard Curve
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Product Details
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Citations (42)
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Human PDGF-BB Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Sensitivity
15 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human PDGF-BB
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 268 639 935 251 655 918
Standard Deviation 11.3 28.4 26.8 22.4 38.5 59.3
CV% 4.2 4.4 2.9 8.9 5.9 6.5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 310 759 1093 287 772 1082
Standard Deviation 14 18.6 25.5 21.9 56.9 89.8
CV% 4.5 2.4 2.3 7.6 7.4 8.3

Recovery

The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 86-102
Platelet-poor EDTA Plasma (n=6) 101 88-115
Platelet-poor Heparin Plasma (n=6) 103 89-112
Serum (n=6) 102 93-114

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PDGF-BB ELISA Linearity

Data Examples

Human PDGF-BB ELISA Cell Culture Supernate Standard Curve

Human PDGF-BB ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:
Platelet-derived Growth Factor BB
Entrez Gene IDs:
5155 (Human); 24628 (Rat)
Alternate Names:
PDGFBB; PDGF-BB
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.5 hours.
    For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human PDGF-BB Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Functional evaluation of PDGFB-variants in idiopathic basal ganglia calcification, using patient-derived iPS cells
    Authors: SI Sekine, M Kaneko, M Tanaka, Y Ninomiya, H Kurita, M Inden, M Yamada, Y Hayashi, T Inuzuka, J Mitsui, H Ishiura, A Iwata, H Fujigasaki, H Tamaki, R Tamaki, S Kito, Y Taguchi, K Tanaka, N Atsuta, G Sobue, T Kondo, H Inoue, S Tsuji, I Hozumi
    Sci Rep, 2019;9(1):5698.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. ?-catenin-activated autocrine PDGF/Src signaling is a therapeutic target in pancreatic cancer
    Authors: TL Kuo, KH Cheng, YS Shan, LT Chen, WC Hung
    Theranostics, 2019;9(2):324-336.
    Species: Human
    Sample Types: Serum
  3. Comparison of the efficacy of cryopreserved human platelet lysate and refrigerated lyophilized human platelet lysate for wound healing
    Authors: SC Notodihard, N Morimoto, N Kakudo, T Mitsui, TM Le, Y Tabata, K Kusumoto
    Regen Ther, 2019;10(0):1-9.
    Species: Human
    Sample Types: Cell Lysates
  4. In vitro fibroblast migration by sustained release of PDGF-BB loaded in chitosan nanoparticles incorporated in electrospun nanofibers for wound dressing applications
    Authors: M Piran, S Vakilian, M Piran, A Mohammadi-, S Hosseinzad, A Ardeshiryl
    Artif Cells Nanomed Biotechnol, 2018;0(0):1-10.
    Species: Human
    Sample Types: Scaffold Supernates
  5. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
    Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
    PLoS ONE, 2018;13(9):e0203048.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels
    Authors: MY Bai, MH Chuang, MF Lin, SL Tang, CC Wong, WP Chan
    Sci Rep, 2018;8(1):10642.
    Species: Rabbit
    Sample Types: Platelet Rich Fibrin
  7. Platelet-rich fibrin prepared from stored whole-blood samples
    Authors: K Isobe, M Suzuki, T Watanabe, Y Kitamura, T Suzuki, H Kawabata, M Nakamura, T Okudera, H Okudera, K Uematsu, K Nakata, T Tanaka, T Kawase
    Int J Implant Dent, 2017;3(1):6.
    Species: Human
    Sample Types: Complex Sample Type
  8. Comparative analyses of industrial-scale human platelet lysate preparations.
    Authors: Jan Pierce, Eric Benedetti, Amber Preslar, Pam Jacobson, Ping Jin, David F Stroncek, Jo-Anna Reems
    Transfusion, 2017;0(0):1537-2995.
    Species: Human
    Sample Types: Plasma
  9. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
    Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
    J Transl Med, 2017;15(1):90.
    Species: Human
    Sample Types: Cell Lysates
  10. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations
    Authors: G Hauschild, F Geburek, G Gosheger, M Eveslage, D Serrano, A Streitbrg, S Johannlke, D Menzel, R Mischke
    BMC Vet. Res, 2017;13(1):7.
    Species: Equine
    Sample Types: Plasma
  11. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Molecular and Microarray-based Analysis of Diversity among Resting and Osteogenically Induced Porcine Mesenchymal Stromal Cells of Several Tissue Origin
    Authors: Samet Bayraktar
    J Tissue Eng Regen Med, 2016;0(0):.
    Species: Porcine
    Sample Types: Plasma
  13. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF)
    Authors: Hideo Masuki
    Int J Implant Dent, 2016;2(1):19.
    Species: Human
    Sample Types: Platelet Rich Plasma (PRP)
  15. Unaltered Angiogenesis-Regulating Activities of Platelets in Mild Type 2 Diabetes Mellitus despite a Marked Platelet Hyperreactivity
    PLoS ONE, 2016;11(9):e0162405.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016;2016(0):6104780.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson's disease patients.
    Authors: Paul G, Zachrisson O, Varrone A, Almqvist P, Jerling M, Lind G, Rehncrona S, Linderoth B, Bjartmarz H, Shafer L, Coffey R, Svensson M, Mercer K, Forsberg A, Halldin C, Svenningsson P, Widner H, Frisen J, Palhagen S, Haegerstrand A
    J Clin Invest, 2015;125(3):1339-46.
    Species: Human
    Sample Types: CSF
  18. Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial.
    Authors: Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, Chiritescu G, Dumon K, D'Hoore A, Gevaert O, Sagaert X, Van Cutsem E, Haustermans K
    Br J Cancer, 2015;112(8):1314-25.
    Species: Human
    Sample Types: Whole Blood
  19. The systemic influence of platelet-derived growth factors on bone marrow mesenchymal stem cells in fracture patients.
    Authors: Tan, Hiang Bo, Giannoudis, Peter V, Boxall, Sally A, McGonagle, Dennis, Jones, Elena
    BMC Med, 2015;13(0):6.
    Species: Human
    Sample Types: Serum
  20. Increased pericyte coverage mediated by endothelial-derived fibroblast growth factor-2 and interleukin-6 is a source of smooth muscle-like cells in pulmonary hypertension.
    Authors: Ricard N, Tu L, Le Hiress M, Huertas A, Phan C, Thuillet R, Sattler C, Fadel E, Seferian A, Montani D, Dorfmuller P, Humbert M, Guignabert C
    Circulation, 2014;129(15):1586-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Temsirolimus inhibits proliferation and migration in retinal pigment epithelial and endothelial cells via mTOR inhibition and decreases VEGF and PDGF expression.
    Authors: Liegl, Raffael, Koenig, Susanna, Siedlecki, Jakob, Haritoglou, Christos, Kampik, Anselm, Kernt, Marcus
    PLoS ONE, 2014;9(2):e88203.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Systemic upregulation of PDGF-B in patients with neovascular AMD.
    Authors: Zehetner C, Kirchmair R, Neururer S, Kralinger M, Bechrakis N, Kieselbach G
    Invest Ophthalmol Vis Sci, 2014;55(1):337-44.
    Species: Human
    Sample Types: Plasma
  23. Regulation of T cell proliferation by JMJD6 and PDGF-BB during chronic hepatitis B infection.
    Authors: Chen C, Feng X, Liao H, Jin W, Zhang J, Wang Y, Gong L, Liu J, Yuan X, Zhao B, Zhang D, Chen G, Wan Y, Guo J, Yan H, He Y
    Sci Rep, 2014;4(0):6359.
  24. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013;14(0):48.
    Species: Human
    Sample Types: Cell Lysates
  25. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
    Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
    Stem Cells, 2013;31(1):146-55.
    Species: Human
    Sample Types: Tissue Homogenates
  26. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008;590(1):400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  27. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
    Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
    Biomaterials, 2008;29(24):3429-37.
    Species: Porcine
    Sample Types: Plasma
  28. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
    Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
    Br. J. Cancer, 2008;99(9):1426-32.
    Species: Human
    Sample Types: Plasma
  29. Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression.
    Authors: Fujii T, Onimaru M, Yonemitsu Y, Kuwano H, Sueishi K
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2785-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
    Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
    Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  31. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
    Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
    Cancer Res., 2007;67(2):555-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
    Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
    Exp. Hematol., 2007;35(7):1119-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
    Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
    Clin. Cancer Res., 2006;12(9):2676-88.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006;169(5):1767-83.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Effects of simvastatin on angiogenic growth factors released at the site of microvascular injury.
    Authors: Undas A, Celinska-Lowenhoff M, Stepien E, Nizankowski R, Tracz W, Szczeklik A
    Thromb. Haemost., 2006;95(6):1045-7.
    Species: Human
    Sample Types: Plasma
  36. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Types: Plasma
  37. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha.
    Authors: Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE
    J. Vasc. Surg., 2005;41(2):321-31.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  39. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice.
    Authors: Saika S, Kono-Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, Muragaki Y, Ooshima A, Yoo J, Flanders KC, Roberts AB
    Lab. Invest., 2004;84(10):1245-58.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1.
    Authors: Zhou W, Park I, Pins M, Kozlowski JM, Jovanovic B, Zhang J, Lee C, Ilio K
    Endocrinology, 2003;144(10):4280-4.
    Species: Human
    Sample Types: Cell Lysates
  42. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
    Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
    Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
    Species: Porcine
    Sample Types: Cell Lysates

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Human PDGF-BB Quantikine ELISA Kit
By Anonymous on 04/09/2018
Sample Tested: Cell Culture Supernates

Human PDGF-BB Quantikine ELISA Kit
By Anonymous on 10/20/2017
Sample Tested: Plasma