• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
  • Sensitivity
    15 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human PDGF-BB
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1418.625.521.956.989.8

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation11.328.426.822.438.559.3


The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 86-102
Platelet-poor EDTA Plasma (n=6) 101 88-115
Platelet-poor Heparin Plasma (n=6) 103 89-112
Serum (n=6) 102 93-114
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PDGF-BB Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-BB
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
    • Long Name
      Platelet-derived Growth Factor BB
    • Entrez Gene IDs
      5155 (Human); 24628 (Rat);
    • Alternate Names
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well.
    10.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.5 hours.
      For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    11.   Aspirate and wash 4 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    32 Citations: Showing 1 - 10
    Filter your results:

    Sample Type
    1. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
      Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
      J Transl Med, 2017;15(1):90.
      Species: Human
      Sample Type: Cell Lysates
    2. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations
      Authors: G Hauschild, F Geburek, G Gosheger, M Eveslage, D Serrano, A Streitbrg, S Johannlke, D Menzel, R Mischke
      BMC Vet. Res, 2017;13(1):7.
      Species: Equine
      Sample Type: Plasma
    3. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
      Stem Cells Int, 2016;2016(0):6104780.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Unaltered Angiogenesis-Regulating Activities of Platelets in Mild Type 2 Diabetes Mellitus despite a Marked Platelet Hyperreactivity
      PLoS ONE, 2016;11(9):e0162405.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
      Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
      J Am Heart Assoc, 2016;5(4):.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
      Sci Rep, 2016;6(0):29029.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. The systemic influence of platelet-derived growth factors on bone marrow mesenchymal stem cells in fracture patients.
      Authors: Tan, Hiang Bo, Giannoudis, Peter V, Boxall, Sally A, McGonagle, Dennis, Jones, Elena
      BMC Med, 2015;13(0):6.
      Species: Human
      Sample Type: Serum
    8. Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial.
      Authors: Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, Chiritescu G, Dumon K, D'Hoore A, Gevaert O, Sagaert X, Van Cutsem E, Haustermans K
      Br J Cancer, 2015;112(8):1314-25.
      Species: Human
      Sample Type: Whole Blood
    9. Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson's disease patients.
      Authors: Paul G, Zachrisson O, Varrone A, Almqvist P, Jerling M, Lind G, Rehncrona S, Linderoth B, Bjartmarz H, Shafer L, Coffey R, Svensson M, Mercer K, Forsberg A, Halldin C, Svenningsson P, Widner H, Frisen J, Palhagen S, Haegerstrand A
      J Clin Invest, 2015;125(3):1339-46.
      Species: Human
      Sample Type: CSF
    10. Systemic upregulation of PDGF-B in patients with neovascular AMD.
      Authors: Zehetner C, Kirchmair R, Neururer S, Kralinger M, Bechrakis N, Kieselbach G
      Invest Ophthalmol Vis Sci, 2014;55(1):337-44.
      Species: Human
      Sample Type: Plasma
    11. Regulation of T cell proliferation by JMJD6 and PDGF-BB during chronic hepatitis B infection.
      Authors: Chen C, Feng X, Liao H, Jin W, Zhang J, Wang Y, Gong L, Liu J, Yuan X, Zhao B, Zhang D, Chen G, Wan Y, Guo J, Yan H, He Y
      Sci Rep, 2014;4(0):6359.
    12. Temsirolimus inhibits proliferation and migration in retinal pigment epithelial and endothelial cells via mTOR inhibition and decreases VEGF and PDGF expression.
      Authors: Liegl, Raffael, Koenig, Susanna, Siedlecki, Jakob, Haritoglou, Christos, Kampik, Anselm, Kernt, Marcus
      PLoS ONE, 2014;9(2):e88203.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Increased pericyte coverage mediated by endothelial-derived fibroblast growth factor-2 and interleukin-6 is a source of smooth muscle-like cells in pulmonary hypertension.
      Authors: Ricard N, Tu L, Le Hiress M, Huertas A, Phan C, Thuillet R, Sattler C, Fadel E, Seferian A, Montani D, Dorfmuller P, Humbert M, Guignabert C
      Circulation, 2014;129(15):1586-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
      Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
      Stem Cells, 2013;31(1):146-55.
      Species: Human
      Sample Type: Tissue Homogenates
    15. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Lysates
    16. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
      Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
      Br. J. Cancer, 2008;99(9):1426-32.
      Species: Human
      Sample Type: Plasma
    17. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
      Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
      Eur. J. Pharmacol., 2008;590(1):400-8.
      Species: Mouse
      Sample Type: Tissue Homogenates
    18. Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression.
      Authors: Fujii T, Onimaru M, Yonemitsu Y, Kuwano H, Sueishi K
      Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2785-91.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
      Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
      Biomaterials, 2008;29(24):3429-37.
      Species: Porcine
      Sample Type: Plasma
    20. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
      Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
      Cancer Res., 2007;67(2):555-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
      Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
      Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    22. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
      Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
      Exp. Hematol., 2007;35(7):1119-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Effects of simvastatin on angiogenic growth factors released at the site of microvascular injury.
      Authors: Undas A, Celinska-Lowenhoff M, Stepien E, Nizankowski R, Tracz W, Szczeklik A
      Thromb. Haemost., 2006;95(6):1045-7.
      Species: Human
      Sample Type: Plasma
    24. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
      Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
      Clin. Cancer Res., 2006;12(9):2676-88.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
      Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
      Am. J. Pathol., 2006;169(5):1767-83.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
      Authors: Herrmann S, Green J, Weber T, Scharfenberger A
      Bone, 2006;39(5):1156-63.
      Species: Human
      Sample Type: Plasma
    27. Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha.
      Authors: Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE
      J. Vasc. Surg., 2005;41(2):321-31.
      Species: Bovine
      Sample Type: Cell Culture Supernates
    28. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
      Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
      J. Cell. Physiol., 2005;203(1):35-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice.
      Authors: Saika S, Kono-Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, Muragaki Y, Ooshima A, Yoo J, Flanders KC, Roberts AB
      Lab. Invest., 2004;84(10):1245-58.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1.
      Authors: Zhou W, Park I, Pins M, Kozlowski JM, Jovanovic B, Zhang J, Lee C, Ilio K
      Endocrinology, 2003;144(10):4280-4.
      Species: Human
      Sample Type: Cell Lysates
    32. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
      Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
      Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
      Species: Porcine
      Sample Type: Cell Lysates
    Expand to show all 32 Citations
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