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Human PDGF-BB Quantikine ELISA Kit

R&D Systems | Catalog # DBB00

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)

Sensitivity

15 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human PDGF-BB Quantikine ELISA Kit

The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human PDGF-BB

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 268 639 935 251 655 918
Standard Deviation 11.3 28.4 26.8 22.4 38.5 59.3
CV% 4.2 4.4 2.9 8.9 5.9 6.5

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 310 759 1093 287 772 1082
Standard Deviation 14.0 18.6 25.5 21.9 56.9 89.8
CV% 4.5 2.4 2.3 7.6 7.4 8.3

Recovery for Human PDGF-BB Quantikine ELISA Kit

The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 86-102
Platelet-poor EDTA Plasma (n=6) 101 88-115
Platelet-poor Heparin Plasma (n=6) 103 89-112
Serum (n=6) 102 93-114

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human PDGF-BB ELISA Linearity

Scientific Data Images for Human PDGF-BB Quantikine ELISA Kit

Human PDGF-BB ELISA Cell Culture Supernate Standard Curve

Human PDGF-BB ELISA Cell Culture Supernate Standard Curve

Human PDGF-BB ELISA Serum/Plasma Standard Curve

Human PDGF-BB ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

PDGF-BB (Platelet-Derived Growth Factor BB) is a homodimeric molecule expressed by hepatocytes and nonresorbing osteoclasts, and it promotes osteoblast development and bone formation. It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization. The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta. PDGF-BB has been shown to bind the alpha/alpha homodimer, alpha/beta heterodimer, and the beta/beta homodimer in vitro, and act through the beta/beta homodimer in vivo.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Gene Symbol

PDGFB

Additional PDGF-BB Products

Product Documents for Human PDGF-BB Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PDGF-BB Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

PDGF Family

Citations for Human PDGF-BB Quantikine ELISA Kit

Customer Reviews for Human PDGF-BB Quantikine ELISA Kit (3)

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  • Human PDGF-BB Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 11/03/2023
    Measured plasma & urine at 2-fold dilution. Concentrations in urine were too low to be detected but the kit worked well for plasma.
    Human PDGF-BB Quantikine ELISA Kit DBB00
  • Human PDGF-BB Quantikine ELISA Kit
    Name: Chloe Warren
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 04/09/2018
    Human PDGF-BB Quantikine ELISA Kit DBB00
  • Human PDGF-BB Quantikine ELISA Kit
    Name: Aileen Rowland
    Sample Tested: Plasma
    Verified Customer | Posted 10/20/2017

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Protocols

View specific protocols for Human PDGF-BB Quantikine ELISA Kit (DBB00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.5 hours.
    For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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