Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta -subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF receptor alpha ‑subunit. Detects the PDGF receptor beta -subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
Monoclonal Mouse IgG1 Clone # PR7212
Protein A or G purified from ascites
Human skin fibroblast membrane extracts
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry.
MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1263N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R beta
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4).
Hart et al. (1987) J. Biol. Chem. 262:10780.
Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
Seifert et al. (1989) J. Biol. Chem. 264:8771.
Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
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