Human PDGF R beta Antibody (PR7212)

R&D Systems | Catalog # MAB1263

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PR7212
View all PDGF R beta Primary Antibodies »

Product Specifications

Immunogen

Human skin fibroblast membrane extracts

Specificity

Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta ‑subunit complexed with 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PDGF R beta Antibody (PR7212)

Detection of PDGF R beta in MG-63 cells by Flow Cytometry

MG-63 cells were stained with Mouse Anti-Human PDGF R beta Monoclonal Antibody (Catalog # MAB1263, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of PDGF R beta by Western Blot

Detection of PDGF R beta by Western Blot

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta, (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PDGF R beta by Western Blot

Detection of PDGF R beta by Western Blot

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta, (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PDGF R beta by Western Blot

Detection of PDGF R beta by Western Blot

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta, (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PDGF R beta by Western Blot

Detection of PDGF R beta by Western Blot

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta, (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PDGF R beta Antibody (PR7212)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: MG-63 human osteosarcoma cell line

Immunoprecipitation

Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.

Western Blot

1 µg/mL
Sample: Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR)

Reviewed Applications

Read 4 reviews rated 4 using MAB1263 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4).

References

  1. Hart et al. (1987) J. Biol. Chem. 262:10780.
  2. Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
  3. Seifert et al. (1989) J. Biol. Chem. 264:8771.
  4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Entrez Gene IDs

5159 (Human); 18596 (Mouse); 24629 (Rat)

Gene Symbol

PDGFRB

Additional PDGF R beta Products

Product Documents for Human PDGF R beta Antibody (PR7212)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PDGF R beta Antibody (PR7212)

For research use only

Citations for Human PDGF R beta Antibody (PR7212)

Customer Reviews for Human PDGF R beta Antibody (PR7212) (4)

4 out of 5
4 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
25%
2 Stars
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1 Stars
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Customer Images

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  • Human PDGF R beta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast Cancer
    Species: Human
    Verified Customer | Posted 09/06/2021
    Human PDGF R beta Antibody (PR7212) MAB1263
  • Human PDGF R beta Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human PDGF R beta Antibody (PR7212) MAB1263
  • Human PDGF R beta Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human PDGF R beta Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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Protocols

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