Human PDGF R beta PE-conjugated Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF R beta -subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF R alpha ‑subunit.
    Detects the PDGF R beta -subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
  • Source
    Monoclonal Mouse IgG1 Clone # PR7212
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Human skin fibroblast membrane extracts
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta PE-conjugated Monoclonal Antibody (Catalog # FAB1263P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt-3 Ligand. All class III RTKs are characterized by the presence of five immunoglobulin‑like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo- and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is a potent PDGF antagonist (4).

  • References:
    1. Hart et al. (1987) J. Biol. Chem. 262:10780.
    2. Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
    3. Seifert et al. (1989) J. Biol. Chem. 264:8771.
    4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
  • Long Name:
    Platelet-derived Growth Factor Receptor beta
  • Entrez Gene IDs:
    5159 (Human); 18596 (Mouse); 24629 (Rat)
  • Alternate Names:
    beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Hypoxia-controlled EphA3 marks a human endometrium-derived multipotent mesenchymal stromal cell that supports vascular growth.
    Authors: To C, Farnsworth R, Vail M, Chheang C, Gargett C, Murone C, Llerena C, Major A, Scott A, Janes P, Lackmann M
    PLoS ONE, 2014;9(11):e112106.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
    Authors: Norton, Kerri-An, Han, Zheyi, Popel, Aleksand, Pandey, Niranjan
    Onco Targets Ther, 2014;7(0):1571-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    , 2014;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB signaling pathway and proinflammatory response in human pericytes.
    Authors: Guijarro-Munoz I, Compte M, Alvarez-Cienfuegos A, Alvarez-Vallina L, Sanz L
    J Biol Chem, 2014;289(4):2457-68.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012;7(10):e47187.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2012;318(4):371-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human PDGF R beta  PE-conjugated Antibody FAB1263P
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Flow Cytometry Human PDGF R beta  PE-conjugated Antibody FAB1263P
Flow Cytometry: Human PDGF R beta PE-conjugated Antibody [FAB1263P]

Summary

ApplicationFlow Cytometry
Sample TestedG55TL Glioblastoma cell line
SpeciesHuman

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