Human PEAR1 PE-conjugated Antibody

FAB4527P has been discontinued. View all PEAR1 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects human PEAR1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Tenascin R or recombinant mouse Tenascin C is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 492621
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human PEAR1
    Accession # Q5VY43
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of PEAR1 in Human Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with anti-human Integrin  alpha 2b/CD41 FITC‑conjugated antibody and either (A) Mouse Anti-Human PEAR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB4527P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PEAR1

Platelet endothelial aggregation receptor 1 (PEAR1) is a 150 kDa type I transmembrane protein and member of the MEGF family of proteins (1). Human PEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20 aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 261 aa cytoplasmic region. The ECD consists of 15 EGF-like repeats that vary in length from 39 to 42 aa and contain a consensus sequence of CX1-2GX2GX2-4CX3CX1-3CX1-2GX1-2CX4GX1CX1CX2GX2GX2C (1). The consensus repeat contains six conserved glycine residues and eight conserved cysteine residues, suggesting four disulfide-bonded cysteine pairs in each EGF repeat (1). Within the ECD, there are also five potential sites for N-linked glycosylation. The cytoplasmic region contains five potential Src homology 3-binding, proline-rich domains (1). Mature human PEAR1 is 84% aa identical to mature mouse PEAR1. PEAR1 is most highly expressed in platelets and endothelial cells (1). Functionally, PEAR1 is a receptor for a yet undetermined ligand that signals upon the formation of platelet‑platelet contacts induced both by platelet aggregations and by platelet centrifugation (1). The signaling enhances and stabilizes platelet thrombi (2). Upon aggregation, the surface-expressed protein is tyrosine-phosphorylated (1). This phosphorylation event is inhibited by the alpha IIb beta 3 antagonist eptifibatide, thus demonstrating that PEAR1 tyrosine phosphorylation is dependent on surface contacts between activated platelets (1). PEAR1 can be phosphorylated in an alpha IIb beta 3 integrin-dependent manner on tyrosine (Tyr925) and serine residues (Ser593 and Ser1029) and, potentially, at Tyr804, Tyr943, and Tyr979 (1). Inherited PEAR1 variations that alter expression or function of the platelet signaling molecule could modify agonist-induced aggregation in native platelets (2). In addition, a genetic variant in PEAR1 could be an important determinant of residual platelet function during aspirin treatment, because the COX1/thromboxane A2 pathway will be strongly inhibited by aspirin, and maximal aggregation will then be dependent on other secondary signaling pathways (2).

  • References:
    1. Nanda, N. et al. (2005) J. Biol. Chem. 280:24680.
    2. Herrera-Galeano, J.E. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1484.
  • Long Name:
    Platelet endothelial aggregation receptor 1
  • Entrez Gene IDs:
    375033 (Human); 73182 (Mouse)
  • Alternate Names:
    Jedi; PEAR1; platelet endothelial aggregation receptor 1
Related Research Areas


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