Human Pepsinogen A5/PGA5 Antibody

Catalog # Availability Size / Price Qty
Detection  of Human Pepsinogen A5/PGA5 by Western Blot.
1 Image
Product Details
Supplemental Products

Human Pepsinogen A5/PGA5 Antibody Summary

Species Reactivity
Detects human Pepsinogen A5/PGA5 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A5/PGA5
Accession # P0DJD7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human Pepsinogen A5/PGA5 by Western Blot. View Larger

Detection of Human Pepsinogen A5/
PGA5 by Western Blot.
Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pepsinogen A5/PGA5

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA-3, PGA-4, and PGA-5 are isozymogens of human Pepsinogen A, which differ in amino acid sequence by 2-4 residues (2). This recombinant human Pepsinogen A corresponds to PGA-4. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

  1. Athauda, S.B. et al. (1989) J. Biochem. 106:920.
  2. Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
Entrez Gene IDs
5222 (Human)
Alternate Names
EC 3.4.23; EC; pepsin A; pepsinogen 5, group I (pepsinogen A); Pepsinogen A5; PGA3; PGA4; PGA5

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Pepsinogen A5/PGA5 Antibody

There are currently no reviews for this product. Be the first to review Human Pepsinogen A5/PGA5 Antibody and earn rewards!

Have you used Human Pepsinogen A5/PGA5 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review