Human Periostin/OSF‑2 Biotinylated Antibody
Human Periostin/OSF‑2 Biotinylated Antibody Summary
Ala22-Gln836
Accession # Q15063
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Periostin/OSF-2
Human OSF-2 (osteoblast-specific factor 2; also periostin) is a 170 kDa secreted homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as an 836 amino acid (aa) precursor that contains a 21 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). The human homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four 130 aa fasciculin type 1 (FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel seven-stranded beta ‑wedge with a multiple alpha -helical fold (1, 8). Three alternate splice forms are known that are C-terminal to the fourfold FAS1 repeats. These mature molecules are 758 and 761 aa in length. The first shows a one aa substitution for aa 649-706 of the mature molecule. The second shows a one aa substitution for aa 649-676, and a deletion of 27 aa between aa 784-810 of the mature molecule (10). The significance of the alternate splice forms is not clear. OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts, osteoblasts, and multiple carcinoma cell types (2, 3, 5, 7, 11, 12). OSF-2 induces expression of VEGFR2/KDR on endothelial cells (EC) by binding to EC alpha v beta 3 (12). It also promotes cell transformation to a tumorigenic phenotype, accompanied by MMP-9 and fibronectin production and cell migration (13). Mature human OSF-2 is 91%, 96% and 91% aa identical to rat, dog, and mouse OSF-2, respectively.
- Clout, N.J. and D. Tisi (2003) Structure 11:197.
- Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
- Gillan, L. et al. (2002) Cancer Res. 62:5358.
- Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
- Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
- Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
- Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
- Takeshita, S. et al. (1993) Biochem. J. 294:271.
- Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
- Swiss-Prot Accession # Q15063.
- Li, G. et al. (2006) Atherosclerosis 188:292.
- Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
- Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
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