Human Phospho-MLKL (T357) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MLKL when phosphorylated at T357.
  • Source
    Monoclonal Mouse IgG2A Clone # 954702
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing the human MLKL T357 site
    Accession # Q8NB16
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Phospho-MLKL (T357) in Human Squamous Cell Carcinoma. MLKLphosphorylated at T357 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Phospho-MLKL (T357) Monoclonal Antibody (Catalog # MAB9187) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MLKL
Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity.  Phosphorylation of MLKL at T357 and S358  by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx.  Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer.  Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.
  • Long Name:
    Mixed Lineage Kinase Domain-like
  • Entrez Gene IDs:
    197259 (Human); 74568 (Mouse); 690743 (Rat)
  • Alternate Names:
    FLJ34389; mixed lineage kinase domain-like protein; mixed lineage kinase domain-like; MLKL
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