Human Phospho-MLKL (T357) Antibody Summary
Accession # Q8NB16
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Phospho-MLKL (T357) in Human Squamous Cell Carcinoma. MLKL phosphorylated at T357 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Phospho-MLKL (T357) Monoclonal Antibody (Catalog # MAB9187) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity. Phosphorylation of MLKL at T357 and S358 by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx. Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer. Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.
Citations for Human Phospho-MLKL (T357) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Excess fructose enhances oleatic cytotoxicity via reactive oxygen species production and causes necroptosis in hepatocytes
Authors: J Kanazawa, K Kakisaka, Y Suzuki, T Yonezawa, H Abe, T Wang, Y Takikawa
The Journal of nutritional biochemistry, 2022;0(0):109052.
Sample Types: Whole Tissue
ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
Authors: R Cheng, X Liu, Z Wang, K Tang
Molecular Medicine Reports, 2021;23(6):.
Sample Types: Cell Lysates
Applications: Western Blot
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