Human Phospho-MLKL (T357) Antibody

Catalog # Availability Size / Price Qty
Phospho-MLKL (T357) in Human Squamous Cell Carcinoma.
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Product Details
Citations (2)
Supplemental Products

Human Phospho-MLKL (T357) Antibody Summary

Species Reactivity
Detects human MLKL when phosphorylated at T357.
Monoclonal Mouse IgG2A Clone # 954702
Protein A or G purified from cell culture supernatant
Phosphopeptide containing the human MLKL T357 site
Accession # Q8NB16
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Phospho-MLKL (T357) antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P). View Larger

Phospho-MLKL (T357) in Human Squamous Cell Carcinoma. MLKL phosphorylated at T357 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Phospho-MLKL (T357) Monoclonal Antibody (Catalog # MAB9187) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MLKL

Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity.  Phosphorylation of MLKL at T357 and S358  by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx.  Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer.  Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.

Long Name
Mixed Lineage Kinase Domain-like
Entrez Gene IDs
197259 (Human); 74568 (Mouse); 690743 (Rat)
Alternate Names
FLJ34389; mixed lineage kinase domain-like protein; mixed lineage kinase domain-like; MLKL

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Citations for Human Phospho-MLKL (T357) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Excess fructose enhances oleatic cytotoxicity via reactive oxygen species production and causes necroptosis in hepatocytes
    Authors: J Kanazawa, K Kakisaka, Y Suzuki, T Yonezawa, H Abe, T Wang, Y Takikawa
    The Journal of nutritional biochemistry, 2022;0(0):109052.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
    Authors: R Cheng, X Liu, Z Wang, K Tang
    Molecular Medicine Reports, 2021;23(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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