The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.
Human Phospho-p53 (S15) Antibody
R&D Systems | Catalog # MAB1839
Discontinued Product
MAB1839 has been discontinued.
View all p53 products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-reported
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 261352
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Product Specifications
Immunogen
Phosphopeptide containing the human p53 S15 site
Accession # P04637
Accession # P04637
Specificity
Detects human p53 that is phosphorylated at S15 in Western blots. In Western blots, no cross-reactivity with human p53 that is unphosphorylated at S15 is detected.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-p53 (S15) Antibody
Detection of Human Phospho-p53 (S15) by Western Blot.
Western blot shows p53 immunoprecipitated from lysates of MCF-7 human breast cancer cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). MCF-7 cell line was untreated (-) or exposed (+) to 10 Gy ionizing radiation (IR) for 3 hours. PVDF membrane was probed with 1-2 µg/mL Mouse Anti-Human Phospho-p53 (S15) Monoclonal Antibody (Catalog # MAB1839) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U ?-phosphatase (?-PPase) for 1 hour. For additional reference the membrane was stripped and reprobed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody HAF1355 (lower panel, Catalog # HAF1355) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of p53 in camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was unstimulated (light orange open histogram) or treated with 1 µM camphtothecin for 6 hours (dark orange filled histogram), then stained with Mouse Anti-Human Phospho-p53 (S15) Monoclonal Antibody (Catalog # MAB1839) or isotype control (Catalog # MAB002, blue open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Applications for Human Phospho-p53 (S15) Antibody
Application
Recommended Usage
CyTOF-reported
Brodie, T.M. et al. (2018) Cytometry Part
A. 93: 406. Ready to be labeled using established
conjugation methods. No BSA or other carrier proteins that could interfere with
conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Camptothecin-treated MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: Camptothecin-treated MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1-2 µg/mL
Sample: Phospho-p53 (S15) immunoprecipitated from lysates of MCF‑7 human breast cancer cell line exposed to 10 Gy ionizing radiation using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355)
Sample: Phospho-p53 (S15) immunoprecipitated from lysates of MCF‑7 human breast cancer cell line exposed to 10 Gy ionizing radiation using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355)
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human Phospho-p53 (S15) Antibody
Product Specific Notices for Human Phospho-p53 (S15) Antibody
For research use only
Citations for Human Phospho-p53 (S15) Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
