|Detection of Human Phospho-Paxillin (Y31) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Phospho-Paxillin (Y31) at approximately 65 to 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Paxillin in HUVEC Human Cells. Paxillin phosphorylated at Y31 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and focal adhesions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
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