Human Phospho-Paxillin (Y31) Antibody

Catalog # Availability Size / Price Qty
MAB61641
MAB61641-SP
Detection of Human Phospho-Paxillin (Y31) by Western Blot.
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Product Details
Citations (2)
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Human Phospho-Paxillin (Y31) Antibody Summary

Species Reactivity
Human
Specificity
Detects Phospho-Paxillin (Y31) in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 698239
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human Paxillin Y31 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Paxillin (Y31) antibody by Western Blot. View Larger

Detection of Human Phospho-Paxillin (Y31) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Phospho-Paxillin (Y31) at approximately 65 to 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Paxillin antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Paxillin in HUVEC Human Cells. Paxillin phosphorylated at Y31 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and focal adhesions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Paxillin

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Entrez Gene IDs
5829 (Human); 19303 (Mouse); 360820 (Rat)
Alternate Names
FLJ16691; Paxillin; PXN

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Citations for Human Phospho-Paxillin (Y31) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Enhanced Dendritic Actin Network Formation in Extended Lamellipodia Drives Proliferation in Growth-Challenged Rac1P29S Melanoma Cells
    Authors: Ashwathi S. Mohan, Kevin M. Dean, Tadamoto Isogai, Stacy Y. Kasitinon, Vasanth S. Murali, Philippe Roudot et al.
    Developmental Cell
  2. Glycine and succinic acid are effective indicators of the suppression of epithelial-mesenchymal transition by fucoxanthinol in colorectal cancer stem-like cells
    Authors: M Terasaki, M Mima, S Kudoh, T Endo, H Maeda, J Hamada, K Osada, K Miyashita, M Mutoh
    Oncol. Rep., 2018-04-24;0(0):.

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